Recover vigorous cells of Magnetospirillum magneticum AMB-1 by capillary magnetic separation |
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Authors: | Jinhua Li Xin Ge Xiaokui Zhang Guanjun Chen Yongxin Pan |
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Institution: | 1. State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, China 2. Biogeomagnetism Group, Paleomagnetism and Geochronology Laboratory (SKL-LE), Institute of Geology and Geophysics, Chinese Academy of Sciences, Beijing, 100029, China 3. Marine College, Shandong University at Weihai, Weihai, 264209, China
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Abstract: | Cultivable magnetotactic bacteria (MTB) in laboratory can provide sufficient samples for molecular microbiological and magnetic
studies. However, a cold-stored MTB strain, such as Magnetospirillum magneticum AMB-1, often loses its ability to synthesize magnetosomes and consequently fails to sense the external magnetic field. It
is therefore important to quickly recover vigorous bacteria cells that highly capable of magnetosome producing. In this study,
a modified capillary magnetic separation system was designed to recover a deteriorating strain of Magnetospirillum magneticum AMB-1 that long-term cold-stored in a refrigerator. The results show that all cells obtained after a 3-cycle treatment were
vigorous and had the ability to produce magnetosomes. Moreover, the 3rd-cycle recovered cells were able to form more magnetosome crystals. Compared with the colony formation method, this new method
is time-saving, easily operated, and more efficient for recovering vigorous MTB cells. |
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