Induction of tributyltin-binding protein type 2 in Japanese flounder, Paralichthys olivaceus, by exposure to tributyltin-d27 |
| |
| Authors: | Nassef Mohamed Tawaratsumita Takahiko Oba Yumi Satone Hina Nakayama Kei Shimasaki Yohei Honjo Tsuneo Oshima Yuji |
| |
| Institution: | a Laboratory of Marine Environmental Science, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan
b Center for Marine Environmental Studies, Ehime University, 2-5 Bunkyo-cho, Matsuyama 790-8577, Japan |
| |
| Abstract: | In this study, individual Japanese flounder were intraperitoneally injected with 2 μg tributyltin-d27 (TBT-d27) fish−1. Blood samples were collected on day 7 after injection. TBT-binding protein types 1 and 2 (TBT-bp1, -bp2) in the blood serum were quantified by western blotting analysis. As a result, the concentration of TBT-bp2 in TBT-d27 treated group increased to 220% of that in the solvent control, whereas the TBT-bp1 concentration decreased to 65% of that in the solvent control. Additionally, a positive relationship between the concentrations of TBT-bp2 and TBT was observed in blood sera of wild and cultured flounder. We suggest that TBT-bp2 is produced in response to TBT exposure and may play an important role in fish physiology. |
| |
| Keywords: | Tributyltin-binding proteins Protein induction |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ |
|
