| 3种硅酸盐矿物粉体对V79和A549细胞的遗传毒性 |
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| 引用本文： | 杨芝芝,张青碧,董发勤,邓建军,霍婷婷,曾娅莉.3种硅酸盐矿物粉体对V79和A549细胞的遗传毒性[J].岩石矿物学杂志,2015,34(6):945-949. |
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| 作者姓名： | 杨芝芝 张青碧 董发勤 邓建军 霍婷婷 曾娅莉 |
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| 作者单位： | 四川医科大学, 四川 泸州 646000;四川医科大学, 四川 泸州 646000;西南科技大学, 四川 绵阳 621000;四川 绵阳四○四医院, 四川 绵阳 621000;西南科技大学, 四川 绵阳 621000;四川 绵阳四○四医院, 四川 绵阳 621000 |
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| 基金项目： | 国家自然科学基金重点资助项目(41130746);国家自然科学基金面上资助项目(41472046) |
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| 摘 要： | 通过噻唑蓝(MTT)比色法、微核试验、单细胞凝胶电泳实验(SCGE),检测了3种硅酸盐矿物粉体(绢云母、石英、钠长石)对V79和A549细胞存活率、微核及DNA损伤的影响,比较和探讨了3种硅酸盐矿物粉体细胞遗传毒性的差异。研究结果表明,3种硅酸盐矿物粉体均不同程度抑制细胞增殖,诱发细胞染色体和DNA损伤,作用大小顺序均为绢云母石英钠长石,且对V79细胞的增殖抑制率和DNA损伤率均明显强于A549细胞,推测一定浓度范围内的硅酸盐矿物粉体对细胞遗传物质的稳定性可能存在影响,对人体可能具有遗传毒性。
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| 关 键 词： | 硅酸盐 矿物粉体 遗传毒性 |
| 收稿时间： | 2014/12/15 0:00:00 |
| 修稿时间： | 2015/9/8 0:00:00 |
The genetic toxicity of three kinds of silicate mineral dusts to V79 and A549 cells |
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| YANG Zhi-zhi,ZHANG Qing-bi,DONG Fa-qin,DENG Jian-jun,HUO Ting-ting and ZENG Ya-li.The genetic toxicity of three kinds of silicate mineral dusts to V79 and A549 cells[J].Acta Petrologica Et Mineralogica,2015,34(6):945-949. |
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| Authors: | YANG Zhi-zhi ZHANG Qing-bi DONG Fa-qin DENG Jian-jun HUO Ting-ting and ZENG Ya-li |
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| Institution: | Sichuan Medical University, Luzhou 646000, China;Sichuan Medical University, Luzhou 646000, China;Southwest University of Science and Technology, Mianyang 621000, China;Sichuan Mianyang 404 Hospital, Mianyang 621000, China;Southwest University of Science and Technology, Mianyang 621000, China;Sichuan Mianyang 404 Hospital, Mianyang 621000, China |
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| Abstract: | In this study, the methyl thiazolyl tetrazolium(MTT) colorimetric assay, micronucleus test and single cell gel electrophoresis(SCGE) were used to detect the per-millage of micronucleus, the survival rate and the degree of DNA damage of three kinds of silicate mineral dusts to V79 and A549 cells which included sericite, quartz and albite. The results show that the three kinds of silicate mineral dusts have different degrees of inhibition of cell proliferation, cell chromosome and DNA damage, with the effect from high to low being sericite >quartz >albite, and the cell proliferation inhibition rate and damage rate of DNA on V79 cells is significantly stronger than those of A549 cells. It is thus inferred that a certain concentration of silicate mineral dusts may affect the stability of the genetic material of cells and the genetic toxicity of the human body. |
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| Keywords: | silicates mineral dusts mineral dusts genetic toxicity |
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