东海表层沉积物中氨氧化微生物群落的空间分布和季节性变化

Ammonia oxidation plays a significant role in the nitrogen cycle in marine sediments. Seasonal and spatial distribution of ammonia-oxidizing archaea(AOA) and betaproteobacteria(β-AOB) in surface sediments from the East China Sea(ECS) were investigated using ammonia monooxygenase α subunit(amo A) gene. In order to characterize the community of AOA and β-AOB, real-time quantitative polymerase chain reaction(q PCR) was carried out in this study, along with environmental parameters. The abundance of β-AOB amo A gene(2.17×106–4.54×107 copy numbers per gram wet weight sediment) was always greater than that of AOA amo A gene(2.18×105–9.89×106 copy numbers per gram wet weight sediment) in all sampling stations. The q PCR results were correlated with environmental parameters. AOA amo A gene copy numbers in April were positively related to temperature and nitrite concentration(p0.05). β-AOB amo A gene copy numbers in August correlated negatively with salinity(p0.01), and correlated positively with ammonium concentration(p0.05). With the increase of salinity, the amo A gene copy ratio of AOB to AOA had a tendency to decrease, which suggested β-AOB dominated in the area of high level ammonium and AOA preferred high salinity area.

Seasonal and spatial distribution of ammonia-oxidizing microorganism communities in surface sediments from the East China Sea

Ammonia oxidation plays a significant role in the nitrogen cycle in marine sediments. Seasonal and spatial distribution of ammonia-oxidizing archaea (AOA) and betaproteobacteria (β-AOB) in surface sediments from the East China Sea (ECS) were investigated using ammonia monooxygenase α subunit (amoA) gene. In order to characterize the community of AOA and β-AOB, real-time quantitative polymerase chain reaction (qPCR) was carried out in this study, along with environmental parameters. The abundance of β-AOB amoA gene (2.17×106-4.54×107 copy numbers per gram wet weight sediment) was always greater than that of AOA amoA gene (2.18×105-9.89×106 copy numbers per gram wet weight sediment) in all sampling stations. The qPCR results were correlated with environmental parameters. AOA amoA gene copy numbers in April were positively related to temperature and nitrite concentration (p <0.05). β-AOB amoA gene copy numbers in August correlated negatively with salinity (p <0.01), and correlated positively with ammonium concentration (p <0.05). With the increase of salinity, the amoA gene copy ratio of AOB to AOA had a tendency to decrease, which suggested β-AOB dominated in the area of high level ammonium and AOA preferred high salinity area.