广西茅尾海红树林土壤放线菌多样性及功能酶活性研究

研究广西茅尾海红树林自然保护区内红树林土壤中可培养放线菌的多样性，挖掘具有高产多种功能酶活性的放线菌类群。应用可培养技术和基于16S rRNA基因序列的系统发育分析研究红树林土壤中可培养放线菌的多样性；并以10种酶活底物为指示物，结合点植法和透明圈法对可培养的放线菌进行功能酶活性筛选。结果显示，从红树林土壤中共分离到444株放线菌，隶属于6目13科24属63种，其中3株放线菌为潜在新种。从63株不同种的放线菌中筛选出56株放线菌在至少1种功能酶活性检测中显示阳性，总阳性率为88.89%；其中具有2种以上功能酶活性的放线菌31株，链霉菌属、微杆菌属和短小杆菌属的产酶菌株最为丰富。综上所述，广西茅尾海红树林自然保护区内红树林土壤中可培养放线菌的种类丰富多样，潜藏着放线菌新资源，且功能酶活性显著，具有较大的挖掘潜力。

Biodiversity and functional enzymes of actinomycetes isolated from mangrove soil in the Maowei Sea,Guangxi

The purpose of this study was to investigate the diversity of actinomycetes collected from the Maowei Sea Natural Reserve of Mangrove in Guangxi,and screen the functional enzymes activities from these actinomycetes.Actinobacterial diversity of Mangrove soil was studied by culturable method and phylogenetic analysis based on 16S rRNA gene sequences.Ten enzyme active substrates were selected as indicator reaction.Activity for functional enzymes was tested by inoculating single colony method.Total of 444 strains of culturable actinomycetes were obtained from mangrove soil environment,they were classified into 63 species,24 genera,13 families and 6 orders by 16S rRNA gene sequence analysis.The three strains of them was potential new species.Streptomyces sp.was the dominant genus.Among them,56 strains were screened with at least one or more enzymes activity.The total positive rate was 88.89%.And two enzymes activity at least could be screened from the 31 strains.And the dominant strains capable of enzyme-producing were of Streptomyces sp.,Microbacterium sp.and Curtobacterium sp.Our results showed that in the Maowei Sea Natural Reserve of Mangrove soil,there existed a higher diversity of culturable actinomycetes,and there are large numbers of unknown actionobacterial groups here.These actinomycetes have the abilities to produce excellent functional enzymes.