| A Fast and Indriect Fluorescent Antibody Assay for the Vibro in Large Yeelow Croaker Pseudosciaena Crocea (Richardson) |
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| 作者姓名： | 王军 鄢庆枇 |
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| 摘 要： | A fast and indirect fluorescnet antibody assay for the Vibrio alginolyticus and V.Parahaemolyticus infecting the large yellow croaker has been developed.The specific antisera for the two strains of vibrio were prepared with New Zealand rabbit and the antiserum and cross-reactive efficacy was tested by coagulation in tube.It showed that the goat anti-rabbit IgG had been labeled by fuorescence isothiocyanate(FITC).The results showed that positive reactions were 100% for the large yellow croaker Pseudosciaena crocea with typical symptom of vibrio infection,while the positive reaction to the pathogen in healthy yellow croakers reached 40%,but seemed negative for aquaculter water.The results demonstrated that this fast and indirect fluorescent antibody assay can be used not only to test the vibrio pathogen in diseased yellow croaker but also in infected animals with no symptom.
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| 关 键 词： | 弧菌 大黄鱼 间接荧光抗体 PseudosciaenaCroces VibrioAlginolyticus VPorahaemolyticus |
| 收稿时间： | 15 January 2002 |
| 修稿时间： | 20 September 2002 |
A fast and indirect fluorescent antibody assay for the vibrio in large yellow croaker<Emphasis Type="Italic">Pseudosciaena crocea</Emphasis> (Richardson) |
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| Wang?Jun,Su?Yongquan,Yan?Qingpi.A Fast and Indriect Fluorescent Antibody Assay for the Vibro in Large Yeelow Croaker Pseudosciaena Crocea (Richardson)[J].Chinese Journal of Oceanology and Limnology,2003,21(1):91-96. |
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| Authors: | Wang Jun Su Yongquan Yan Qingpi |
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| Institution: | (1) Dept. of Oceanography & Institute of Subtropical Ocean, Xiamen University, 361005 Xianmen, China;(2) Institute of Aquaculture Biotechnology & Fishery College, Jimei university, 361021 Xiamen, China |
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| Abstract: | A fast and indirect fluorescent antibody assay for theVibrio alginolyticus andV. parahaemolyticus infecting the large yellow croaker has been developed. The specific antisera for the two strains of vibrio were prepared
with New Zealand rabbit and the antiserum and cross-reactive efficacy was tested by coagulation in tube. It showed that the
goat anti-rabbit IgG had been labeled by fluorescence isothiocyanate (FITC). The results showed that positive reactions were
100% for the large yellow croakerPseudosciaena crocea with typical symptom of vibrio infection, while the positive reaction to the pathogen in healthy yellow croakers reached
40%, but seemed negative for aquaculture water. The results demonstrated that this fast and indirect fluorescent antibody
assay can be used not only to test the vibrio pathogen in diseased yellow croaker but also in infected animals with no symptom.
Project supported by the National High-Tech. Development plan of China (863-819-02-12). |
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| Keywords: | Pseudosciaena crocea Vibrio alginolyticus V Parahaemolyticus indirect fluorescent antibody |
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