Isolation, cloning and sequencing of AFLP markers related to disease-resistance traits in Fenneropenaeus chinensis |
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| Authors: | Yue Zhiqin Wang Weiji Kong Jie Dai Jixun |
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| Affiliation: | (1) Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Yellow Sea Fisheries Research Institute, 266071 Qingdao, China;(2) College of Marine Life Science, Ocean University of China, 266003 Qingdao, China;(3) Food Laboratory of qingdao Entry-Exit Inspection and Quarantine Bureau, 266001 Qingdao, China |
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| Abstract: | Amplified fragment length polymorphism (AFLP) technique was used to analyze the fingerprinting of four successive generations ofFenneropenaeus chinensis to reveal their disease-resistance traits. Some loci showed quite different genetic frequencies due to artificial selection, which implied that these fragments were putative markers related to the disease-resistance trait. We developed a simple and effective method to further characterize these AFLP fragments. Specific AFLP bands were cut directly from polyacrylamide gels, re-amplified, cloned and sequenced. Eight putative genetic markers were sequenced and their sizes ranged from 63 to 209 bp. The sequences were submitted to dbGSS (database of Genome Sequence Survey); and the BLAST analysis showed low similarity to the function genes, indicating these markers were tightly linked to a disease-resistance trait but were not functional genes. This research was supported by special funds from the National Key Basic Research Program (G1999012007) and the National High-Tech Research and Development Program of China (863 Program. 2001AA620105). |
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| Keywords: | AFLP Fenneropenaeus chinensis disease-resistance trait markers clone sequence |
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