Estimation of spawning area of <Emphasis Type="Italic">Pisodonophis sangjuensis</Emphasis> Ji and Kim, 2011 (Pisces: Ophichthidae) based on leptocephali size and distribution

Pre-metamorphic leptocephali of Pisodonophis sangjuensis (n = 91, 10.4?90.2 mm in total length, TL) were collected in the East China Sea for the first time. Pre-metamorphic leptocephali of P. sangjuensis, which were identified using mitochondrial DNA cytochrome c oxidase subunit I gene (mtDNA COI), are characterized by various combinations of morphological characters: 8 moderate to pronounced gut loops with the kidney terminating on the 6-7th loops; and 8 subcutaneous pigment patches on the tail just ventral to the notochord. Pisodonophis sangjuensis leptocephali were more numerously collected offshore than inshore around Jeju Island. The smallest leptocephali (< 15.0 mm TL) were collected from the south far from Jeju Island, and the largest leptocephali (> 100.0 mm TL) were collected from around Jeju Island and the southern coast of Korea. Our findings indicate that P. sangjuensis spawns offshore south of Jeju Island that is an area associated with high water temperature, and then the hatched leptocephali are transported to Jeju Island or the southern coast of Korea by the Tsushima Warm Current.