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黄、东海平滑真刺水蚤种群遗传结构的ISSR分析
引用本文:刘光兴,陈晓峰,崔建丽.黄、东海平滑真刺水蚤种群遗传结构的ISSR分析[J].中国海洋大学学报(自然科学版),2009,39(5).
作者姓名:刘光兴  陈晓峰  崔建丽
作者单位:1. 中国海洋大学海洋环境与生态教育部重点实验室,山东,青岛,266003
2. 中国海洋大学海洋环境与生态教育部重点实验室,山东,青岛,266003;中国海洋大学水产学院,山东,青岛,266003
基金项目:国家重点基础研究发展计划项目(2005CB422306)资助
摘    要:为探求黄东海平滑真刺水蚤(Euchaeta plana)是否存在分化,对南黄海、东海2个海域平滑真刺水蚤进行ISSR分析。利用筛选出的4个ISSR引物对平滑真刺水蚤进行PCR扩增。共获得18个扩增位点,其中多态性位点14个,所占比例为77.78%。利用POPGENE软件计算遗传距离为0.071 8,遗传相似系数为0.930 7,从而说明2个群体之间遗传分化很小,并推断为黄海暖流影响其分布的结果。

关 键 词:平滑真刺水蚤  ISSR分析  遗传多样性  群体  

Analysis of Population Genetic Structure of Euchaeta plana with ISSR Technology in Yellow Sea and East China Sea
LIU Guang-Xing,CHEN Xiao-Feng,CUI Jian-Li.Analysis of Population Genetic Structure of Euchaeta plana with ISSR Technology in Yellow Sea and East China Sea[J].Periodical of Ocean University of China,2009,39(5).
Authors:LIU Guang-Xing  CHEN Xiao-Feng    CUI Jian-Li
Institution:Ocean University of China 1.Key Laboratory of Marine Environment and Ecology;Ministry of Education;2.College of Fisheries;Qingdao 266003;China
Abstract:ISSR(inter simple sequence repeat) molecular markers were used to investigate the degree of genetic differentiation between two populations of Euchaeta plana from southern Yellow Sea and East China Sea.Four polymorphic primers were selected to amplify the DNA of Euchaeta plana A total of 18 loci were amplified,of which 14(77.78%) were polymorphic.POPGENE software was used to calculate the genetic diversity indices and the data showed that the genetic distance and genetic similarity between the two populatio...
Keywords:Euchaeta plana  ISSR analysis  genetic diversity  population  
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