超声波对湛江等鞭金藻生长和脂肪酸组成的影响

设计了一个超声频率、超声功率和处理时间三因素四水平的正交实验,研究了超声波对湛江等鞭金藻的生物学效应.实验结果表明,超声频率对湛江等鞭金藻的生物学效应显著,而超声功率和处理时间的影响相对较小.对于提高湛江等鞭金藻生长速率常数,最佳超声条件为20kHz,6W,10s3次;对于提高其脂肪酸不饱和度和主要不饱和脂肪酸百分比,最佳超声条件为20kHz,4～6W,30s.在最佳超声条件下,湛江等鞭金藻生长速率常数最高可达0.630d,是对照组的2.02倍,其脂肪酸不饱和度最高可达79.6%,比对照组提高7.8%,其中主要不饱和脂肪酸的百分含量均得到不同程度的提高.

Effects of ultrasonic wave on the growth and fatty acid composition of Isochrysis zhanjiangensis

A three-factors and four-levels orthogonal experiment,including ultrasonic frequency,power and treatment duration,is conducted to study biological effect of ultrasonic treatment on Isochrysis zhanjiangensis.The experimental results suggest that ultrasonic frequency has significant biological effect,and ultrasonic power and treatment duration have less influence on this alga.The optimum ultrasonic conditions are 20 kHz,6 W,10 s (triplicate) for increasing the constant of growth rate of this alga,and 20 kHz,4~6 W,30 s for increasing its unsaturation degree of total fatty acids(TFA) and the percentages of major unsaturated fatty acids.The maximum constant of growth rate of Isochrysis zhanjiangensis can be 0.630 d which is 2.02 times of that of the controls.The maximum unsaturation degree can be 79.6% which was 7.8% higher than that of the controls.The percentages of major unsaturated fatty acids in TFA can increase more or less under the optimum ultrasonic conditions.