九孔鲍养殖水体中产酶菌群的研究

为了解九孔鲍养殖环境中细菌生理菌群的生态作用,对分离自养殖水体的33株菌株的产酶能力和种类进行了研究。结果表明,具有分泌胞外蛋白酶、脂肪酶和纤维素酶能力的菌株分别为39.4%、57.6%和30.3%;在这33株菌中,产至少一种胞外酶的菌株比例高达63.6%。API鉴定结果表明,在有能力分泌胞外蛋白酶、脂肪酶和纤维素酶的菌株中,弧菌菌群所占的比例分别为46%、42%和40%,而其中河流弧菌又占各弧菌菌群比例的83.3%、62.5%和100%。充分阐明弧菌,尤其是河流弧菌为优势菌,在鲍鱼养殖微生物态中起的重要作用。

Studies on Extra-cellular Enzymes Producing Bacteria in Farming Waters of Abalone Haliotis diversicolor

To understand the ecological roles of bacterial flora in the abalone farming waters, 33 strains of bacteria were isolated and their extra-cellular enzyme producing capabilities were investigated. Results showed that the proportion of bacterial strains which produced extra-cellular protease, lipase and cellulase were at 39.4%,57.6% and 30.3% respectively. Among them, 63.6% strains were able to produce one or more extra-cellular enzymes. To better understand the make-up of the enzyme producing strains, API 20E identification system were employed and results indicated that majority of the enzyme producing strains were vibrios. They made up 46%, 42% and 40% of the protease, lipase and cellulase producing populations respectively. Furthermore, API analysis also revealed that Vibrio fluvialis was the dominant species in enzyme producing vibrios, made up to 83.3%, 62.5% and 100% of the protease, lipase and cellulase producing vibrio population correspondingly. This study fully demonstrated the ecological importance of vibrios, in particular Vibrio fluvialis in abalone aquaculture.