浒苔多糖的分级纯化及保湿活性研究

用DEAE-Sepharose FF离子交换凝胶层析柱对浒苔(Enteromorpha)多糖进行分级纯化,利用HPLC色谱分析了单糖组成,并以甘油和透明质酸为对照,研究其吸湿和保湿活性。结果表明,浒苔多糖主要为葡萄糖醛酸-鼠李糖-木糖-甘露糖聚合物;浒苔多糖主要由两种组分组成,其一组分木糖和鼠李糖含量摩尔比为1.11:1,另一组分木糖和鼠李糖含量比为1:3.28;浒苔多糖具有与透明质酸相似的吸湿和保湿能力。

Polysaccharides from Enteromorpha linza: Purification and moisture-preserving activity

To study the properties of moisture absorption and retention by polysaccharides from Enteromorphalinza. The polysaccharides were purified by anion-exchange chromatography (a DEAE-Sepharose FF column). The monosaccharide residues were analyzed by HPLC (SHIMADZU-20A). The properties of moisture absorption and retention of polysaccharides from Enteromorpha linza were investigated by controlling the relative humidity, and compared with glycerol and hyaluronic acid. The main composition of the polysaccharides was glucurono-xylomannorhamnan. The polysaccharides had two sub-fractions; the ratio of xylose/rhamnose for one fraction was 1.1:11, and 1:3.28. for the other. The polysaccharides and hyaluronic acid had similar properties of moisture absorption and retention. The Polysaccharides from Enteromorpha linza had distinct moisture-preserving activity, and can be further studied as a type of natural moisturizer.