An improved method for the determination of phytoplankton chlorophyll using N,N-dimethylformamide |
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| Authors: | Reiko Suzuki Takashi Ishimaru |
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| Affiliation: | (1) Ocean Research Institute, University of Tokyo, Nakano-ku, 164 Tokyo, Japan;(2) Tokyo University of Fisheries, Konan, Minato-ku, 108 Tokyo, Japan |
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| Abstract: | Non volatile N, N-dimethylformamide (DMF) at room temperature is proposed as a new solvent for determining the chlorophylla in cultured and natural phytoplankton samples. Advantages of DMF compared to the solvents ordinarily used are (1) higher extraction efficiency, (2) rapid extraction time of 30 min, (3) simple extraction procedure requiring neither extra heating nor grinding, but soaking, and (4) a long stable life of chlorophylla (at least one month at 5°C in the dark). |
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