基于微卫星的泥蚶5个地理群体遗传多样性分析

为了研究泥蚶不同地理群体的系统发育关系和遗传多样性,运用微卫星DNA标记,对浙江温州(ZJ)、山东日照(SD)、韩国釜山(KR)、广西企沙(GX)、海南海口(HN)等5个泥蚶地理群体进行了17个基因位点的遗传多样性分析。结果显示,从17对引物中共检测出115个等位基因,每个位点等位基因数(Na)2~12个。有效等位基因数(Ne)为1.192~7.849,平均观测杂合度(Ho)为0.430~0.516,平均期望杂合度(He)为0.573~0.656,5个群体的平均多态信息含量(PIC)为0.525~0.608。Hardy-Weinberg平衡检验表明,51.2%的微卫星位点偏离平衡状态(P0.05)。5个群体间的种群遗传分化系数(FST)为0.012~0.062,呈现出较低的遗传分化。UPMGA聚类分析表明,浙江群体和韩国群体首先聚在一起,亲缘关系最近,然后与海南群体聚合;广西群体和山东群体亲缘关系较近,聚为一支,然后与上面三个群体聚合。

Microsatellite analysis of genetic diversity in five geographic populations of Tegillarca granosa

Genetic diversity was analyzed in five geographic populations of Tegillarca granosa by using 17 polymorphic microsatellite markers. The five different populations were sampled in Wenzhou City, Zhejiang Province; Rizhao City, Shandong Province; Qisha City, Guangxi Province; Haikou City, Hannan Province and Korea. In the seventeen microsatellite markers, 115 alleles were found and allele number per locus was 2-12. Mean effective allele number, mean observed and expected heterozygosity, and mean polymorphism information content were 1.192-7.849, 0.430-0.516 and 0.573-0.656 and 0.525-0.608, respectively. The Hardy-weinberg balance test showed that 51.2% microsatellite sites deviated from equilibrium(P< 0.05). The FST value of the five geographic populations ranged from 0.012 to 0.062, revealing a low level of genetic differentiation among them. The UPMGA tree indicated that Zhejiang and Korea populations clustered firstly, then clustered with Hainan population, and the Guangxi population clustered with Shandong population into another clade.