Evaluating the use of 3'-(p-Aminophenyl) fluorescein for determining the formation of highly reactive oxygen species in particle suspensions |
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| Authors: | Corey A Cohn Christopher E Pedigo Shavonne N Hylton Sanford R Simon Martin AA Schoonen |
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| Affiliation: | (1) Center for Environmental Molecular Science, Stony Brook University, Stony Brook, NY 11794-2100, USA;(2) Department of Geosciences, Stony Brook University, Stony Brook, NY 11794-2100, USA;(3) Department of Biological Sciences, Stony Brook University, Stony Brook, NY 11794, USA;(4) Department of Pathology, Stony Brook University Hospital, Stony Brook, NY 11794, USA;(5) National Research Centre for the Working Environment, Lerso Parkalle 105, 2100 Copenhagen, Denmark |
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| Abstract: | Background Given the importance of highly reactive oxygen species (hROS) as reactants in a wide range of biological, photochemical, and environmental systems there is an interest in detection and quantification of these species. The extreme reactivity of the hROS, which includes hydroxyl radicals, presents an analytical challenge. 3'-(p-Aminophenyl) fluorescein (APF) is a relatively new probe used for measuring hROS. Here, we further evaluate the use of APF as a method for the detection of hydroxyl radicals in particle suspensions. |
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