北极海洋放线菌Streptomyces sp.604F中的抗霉素A和伏尔加霉素类成分

极地微生物代谢产生芳香族硝基化合物、肽类、萜类等多种骨架类型的化学成分。为从北极放线菌中发现结构新颖、活性显著的小分子化合物,本研究采用Sephadex LH-20凝胶和半制备HPLC等柱层析分离纯化化合物手段,结合LC-MS、1H-和13C-NMR等波谱学数据和文献对比进行结构解析,从链霉菌Streptomyces sp.604F提取物中分离鉴定出抗霉素A1b(1)、抗霉素A4a(2)、抗霉素A2a(3)、抗霉素A3a(4)、抗霉素A1a(5)以及伏尔加霉素(6)和8-脱氧伏尔加霉素(7)这7个化合物。化合物1—7是首次从北极海洋沉积物来源的微生物中发现,而且是首次从同一菌株中发现抗霉素A和伏尔加霉素这两类化合物。

Antimycin A and Vulgamycin Derivatives from the Arctic Marine Actinomycete Streptomyces sp. 604F

Various types of constituents, including aromatic nitro compounds, peptides, and terpenoids, have been reported from different polar microorganisms. In our investigation of secondary metabolites of Arctic actinomycete with new structures or potent bioactivity, five antimycin A derivatives, Antimycin A1b (1), Antimycin A4a (2), Antimycin A2a (3), Antimycin A3a (4), Antimycin A1a (5), together with Vulgamycin (6) and its derivative 8-deoxyenterocin (7), were obtained from Streptomyces sp. 604F. The compounds were purified by Sephadex LH-20 column chromatography and semi-preparative high-performance liquid chromatography, and their structures were determined by comparing their liquid chromatograph-mass spectrometry and ¹H- and ¹³C-NMR data with those of previously reported compounds. This is the first report of compounds 1—7 from microbes derived from Arctic sediment, and it is the first time that antimycin and vulgamycin compounds were identified as metabolites from the same source.