| 96孔酶标板法测定中国对虾血淋巴上清液抗菌活力和酚氧化酶活性的初步研究 |
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| 引用本文： | 雷质文,黄捷,杨冰,张立敬,俞开康.96孔酶标板法测定中国对虾血淋巴上清液抗菌活力和酚氧化酶活性的初步研究[J].海洋湖沼通报,2001(4):33-37. |
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| 作者姓名： | 雷质文 黄捷 杨冰 张立敬 俞开康 |
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| 作者单位： | 1. 中国水产科学研究院黄海水产研究所,青岛,266071:
2. 青岛海洋大学水产学院,青岛,266003 |
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| 摘 要： | 用96孔酶标板法测量单尾中国对虾血淋巴上清液抗菌（Uα）应力和过氧化酶活性。实验表明，该法具有以下优点：1）可测定大批单尾对虾血淋巴上清液的抗菌活力，酚氧化酶活性；2）所需对虾血淋巴上清液微量化；3）用酶标仪读取酶的活性，减少了不同试验者人为读数时的误差；4）重复性高。
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| 关 键 词： | 96孔酶标板 中国对虾 血淋巴上清液 抗菌活力 甲壳运物 免疫机制 活氧化酶活性 |
| 文章编号： | 1003-6482(2001)04-0033-05 |
| 修稿时间： | 2001年3月22日 |
A 96-MICROTITOR PLATE METHOD FOR DETECTION OF ANTIMICROBIAL ACTIVITY AND PHENOLOXIDASE ACTIVITY OF HAEMOLYMPH SUPERNATANT OF PENAEUS CHINENSIS |
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| LEI Zhiwen HUANG Jie,YANG Bing ZHANG Lijing YU Kaikang.A 96-MICROTITOR PLATE METHOD FOR DETECTION OF ANTIMICROBIAL ACTIVITY AND PHENOLOXIDASE ACTIVITY OF HAEMOLYMPH SUPERNATANT OF PENAEUS CHINENSIS[J].Transaction of Oceanology and Limnology,2001(4):33-37. |
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| Authors: | LEI Zhiwen HUANG Jie YANG Bing ZHANG Lijing YU Kaikang |
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| Institution: | LEI Zhiwen1 HUANG Jie1 YANG Bing1 ZHANG Lijing YU Kaikang2 |
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| Abstract: | A 96-microtitor plate method for the detection of the antimicrobial activity and phenoloxidase activity of haemolymph supernatant of Penaeus chinensis is presented. 1) This method may be used to detect the antimicrobial activity and phenoloxidase activity of haemolymph supernatant of many single shrimp.2) The volume of haemolymph supernatant of single shrimp required in the tests is very small. 3) Asa 96-microtitor plate reader is used, the artificial error on data read by different researchers can be reduced. 4)The repeatability of the results is very good. |
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| Keywords: | Microtitor plate Penaeus chinensis Haemolymph supernatant Antimicrobial activity Phenoloxidase activity |
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