| 锯缘青蟹(Scylla serrata)特异性过敏原的研究 |
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| 引用本文： | 宋益银,林 蕾,苏秀榕,王春琳,张春丹.锯缘青蟹(Scylla serrata)特异性过敏原的研究[J].海洋与湖沼,2009,40(1):62-67. |
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| 作者姓名： | 宋益银 林 蕾 苏秀榕 王春琳 张春丹 |
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| 作者单位： | 1. "应用海洋生物技术"教育部重点实验室,宁波大学生命科学与生物工程学院,宁波,315211
2. 宁波大学医学院附属医院,宁波,315020 |
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| 基金项目： | 浙江省重大科研项目，浙江省教育厅重点项目，浙江省重中之重学科资助项目 |
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| 摘 要： | 采用SDS聚丙烯酰胺凝胶电泳方法,对锯缘青蟹肌肉中可溶性蛋白质进行分离,以过敏患者阳性血清和正常人阴性血清作对照,通过Western blot鉴定出分子量为14.3kDa的蛋白为锯缘青蟹的特异性过敏原.然后用Sephadex G75凝胶过滤层析和DEAE-52离子交换层析纯化出该过敏原,再经MALDI-TOF/TOF-MS检测,利用Blastz在线分析.结果表明,锯缘青蟹特异性过敏原为血蓝蛋白亚基,经Clustal W分析该蛋白的多肽片段序列与可口美青蟹、邓杰内斯蟹、加州龙虾、艾氏真蟹的血蓝蛋白亚基序列有很高的同源性,经Prosite数据库检索后发现肽段1和肽段2有酪蛋白激酶Ⅱ磷酸化位点和蛋白激酶C磷酸化位点,是过敏原参与机体反应的重要活性位点.
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| 关 键 词： | 锯缘青蟹过敏原 蛋白免疫印迹 层析 质谱分析 |
| 收稿时间： | 2007/12/17 0:00:00 |
| 修稿时间： | 2008/1/29 0:00:00 |
THE SPECIFIC ALLERGENS IN CRAB SCYLLA SERRATA |
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| SONG Yi-Yin,LIN Lei,SU Xiu-Rong,WANG Chun-Lin and ZHANG Chun-Dan.THE SPECIFIC ALLERGENS IN CRAB SCYLLA SERRATA[J].Oceanologia Et Limnologia Sinica,2009,40(1):62-67. |
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| Authors: | SONG Yi-Yin LIN Lei SU Xiu-Rong WANG Chun-Lin and ZHANG Chun-Dan |
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| Institution: | 1.Key Laboratory of Applied Technology of Marine Biology;Ministry of Education;Faculty of Life Science and Biotechnology;Ningbo University;Ningbo;315211;2.Affiliated Hospital of Medical School;315020 |
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| Abstract: | To study the specific allergen in crab Scylla serrata,soluble protein from the muscle of S.serrata was extracted,and then separated by SDS-PAGE.With the positive and negative sera of anaphylactic patients,the special allergen was identified with the Western Blot,showing that the 14.3kDa was the specific allergen.The protein of 14.3kDa was then analyzed by gel filtration in ion exchange chromatography,and the MALDI-TOF/TOF-MS search on the Internet.The results indicate that the protein was hemocyanin subunit... |
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| Keywords: | SDS-PAGE |
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