| 鲢鱼降血压肽的酶解条件 |
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| 引用本文： | 毋瑾超,汪依凡,方长富. 鲢鱼降血压肽的酶解条件[J]. 广东海洋大学学报, 2006, 26(1): 36-39 |
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| 作者姓名： | 毋瑾超 汪依凡 方长富 |
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| 作者单位： | 国家海洋局第二海洋研究所,浙江,杭州,310012 |
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| 基金项目： | 国际科学基金IFS和OPCW资助项目(E/3352-1) |
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| 摘 要： | 以鲢鱼蛋白为原料,采用5种不同复合蛋白酶(P1,P2,P3,P4,P5)进行酶解,综合研究了复合生物酶酶量、酶解时间、pH、酶解温度等对鲢鱼蛋白酶解降血压肽ACE活性抑制效果的影响。结果表明,复合酶P1酶解降血压肽对ACE抑制率经体外检测为88.08%,具有最佳的酶解效果。对应最佳条件为:酶量25 IU/mL,酶解时间2 h,pH7.0,酶解温度60℃。
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| 关 键 词： | 降血压肽 酶解 ACE抑制活性 鲢鱼 |
| 文章编号： | 1007-7995(2006)01-0036-04 |
| 修稿时间： | 2005-07-08 |
Study on Manufacture of Enzymolyzed Antihypertensive Peptide Abstracted from Silver Carp |
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| WU Jin-chao,WANG Yi-fan,FANG Chang-fu. Study on Manufacture of Enzymolyzed Antihypertensive Peptide Abstracted from Silver Carp[J]. Journal of Zhanjiang Ocean University, 2006, 26(1): 36-39 |
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| Authors: | WU Jin-chao WANG Yi-fan FANG Chang-fu |
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| Abstract: | This paper deals with the effects of the quantity of enzyme concentration,hydrolysis temperature,pH and hydrolysis duration of 5 different complex enzymes on the ACE(Angiotensin-converting enzyme) inhibitory activity of antihypertensive peptide abstracted from silver carp.The results are as follows: enzyme P1 has the best hydrolysis result,and the ACE inhibitory ratio of the antihypertensive peptide is 88.08% in vitro.The suitable hydrolysis conditions of this certain complex enzyme are given with enzyme concentration of 25 IU/mL,hydrolysis duration of 2 h,pH of 7.0,hydrolysis temperature of 60 ℃. |
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| Keywords: | antihypertensive peptide enzymolysis ACE inhibitory activity silver carp |
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