Chromatographic studies of dissolved organic matter and copper-organic complexes isolated from estuarine waters |
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| Authors: | Gary L Mills Eileen McFadden James G Quinn |
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| Affiliation: | 1. Department of Environmental Engineering, National I-Lan University, No. 1 Sheen-Long Road, I-Lan City 260, Taiwan;2. College of the Environment and Ecology, Xiamen University, South Road of Xiangan, Xiamen 361102, Fujian, China;3. Graduate Institute of Environmental Engineering, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, 10617 Taipei, Taiwan;1. Advanced Membrane Technology Research Centre (AMTEC), Universiti Teknologi Malaysia, 81310 Johor Bahru, Johor, Malaysia;2. School of Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia, 81310 Johor Bahru, Johor, Malaysia;3. Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310 Johor Bahru, Johor, Malaysia;4. Department of Chemistry, Yobe State University, Damaturu, Yobe State, Nigeria;5. Department of Chemistry, Faculty of Education, University of Sulaimani, Kurdistan, Iraq;1. School of Geography, School of Environment, Nanjing Normal University, Nanjing, 210023, China;2. Key Laboratory of Virtual Geographic Environment (Nanjing Normal University), Ministry of Education, Nanjing, 210023, China;3. Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and Application, Nanjing, 210023, China;4. State Key Laboratory of Pollution Control and Resources Reuse, School of the Environment, Nanjing University, Nanjing, 210023, China;1. Departamento de Investigación en Polímeros y Materiales, Universidad de Sonora, Blvd. Luis Encinas Johnson y Rosales S/N, 83000, Hermosillo, Sonora, México;2. CONACYT- Universidad de Sonora, Blvd Luis Encinas Johnson y Rosales S/N, 83000, Hermosillo, Sonora, México;3. Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, Ciudad Universitaria, 72570, Puebla, Puebla, México |
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| Abstract: | Dissolved organic matter (DOM) and dissolved copper-organic complexes were isolated from the estuarine waters of Narragansett Bay, RI, using reverse-phase liquid chromatography (RPLC). Different types of reverse-phase BOND ELUT columns (Analytichem International), including C2, C18 and phenyl-bonded phases, were studied to determine their adsorption efficiency for extracting DOM. Extraction efficiencies followed the order phenyl > C18 > C2, and phenyl − C18 > C2 for DOM and organic copper, respectively. However, comparisons of BOND ELUT and C18 SEP-PAK (Waters Associates) columns indicated that SEP-PAK columns were the most efficient when both DOM and organic copper were considered. Chromatographic profiles of the isolated DOM obtained using high-performance liquid chromatography were similar in elution characteristics and resembled chromatograms typical of fulvic acid. The UV-absorption characteristics of the DOM showed small differences and suggested that the different reverse-phase columns isolated material that was qualitatively similar.Copper-organic complexes isolated using C18 RPLC were studied to examine the dissociation of organically bound copper in seawater as the pH is lowered. Only a small amount of the complexed copper was displaced by the H+ with about 40% of the copper remaining bound at pH 3. However, the chromatographic elution behavior of the DOM and organic copper was significantly altered under acidic conditions as a result of protonation of acidic functional sites on the organic matter. |
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