| Stress Responses to Rapid Temperature Changes of the Juvenile Sea Cucumber （Apostichopusjaponicus Selenka） |
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| 作者姓名： | DONG Yunwei JI Tingting DONG Shuanglin |
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| 作者单位： | The Key Laboratory of Mariculture, Ministry of Education of P. R. China Qingdao 266003, P. R. China |
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| 基金项目： | 国家自然科学基金;国家重点科技研究发展项目 |
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| 摘 要： | Activities of hexokinase （HK）, pyruvate kinase （PK）, superoxide dismutase （SOD） and catalase （CAT） and Hsp70 level were measured to evaluate the response of the commercially important sea cucumber （Apostichopus japonicus Selenka） to rapid temperature changes in laboratory. Animals were subjected to a higher temperature （from 10 to 20℃） （Tinc treatment） or to a lower temperature （from 20 to 10℃） （Tddec treatment） for 72 h. At 1, 3, 12, 24, 72 h of exposure, animals were removed and prepared for further analysis. Results showed that the effect of acute temperature changes on enzyme activities was significant. In Tinc treatment, activities of SOD and CAT increased immediately. The significant enhancement of SOD and CAT activities suggested that oxidative stress increases significantly when ambient temperature increasing from 10 to 20℃. The up-regulation of Flsp70 in Tinc and Tdec treatments indicated that Hsp70 was a bioindicator of thermal stress in the sea cucumber, and the expression pattern depended on the thermal treatment.
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| 关 键 词： | 抗氧化剂 酶学 己糖激酶 海洋植物 |
| 收稿时间： | 18 July 2006 |
| 修稿时间： | 2006-07-182007-01-05 |
Stress responses to rapid temperature changes of the juvenile sea cucumber (<Emphasis Type="Italic">Apostichopus japonicus</Emphasis> Selenka) |
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| DONG Yunwei JI Tingting DONG Shuanglin.Stress responses to rapid temperature changes of the juvenile sea cucumber (Apostichopus japonicus Selenka)[J].Journal of Ocean University of China,2007,6(3):275-280. |
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| Authors: | Dong Yunwei Ji Tingting Dong Shuanglin |
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| Institution: | The Key Laboratory of Mariculture, Ministry of Education of P. R. China Qingdao 266003, P. R. China |
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| Abstract: | Activities of hexokinase (HK), pyruvate kinase (PK), superoxide dismutase (SOD) and catalase (CAT) and Hsp70 level were measured
to evaluate the response of the commercially important sea cucumber (Apostichopus japonicus Selenka) to rapid temperature changes in laboratory. Animals were subjected to a higher temperature (from 10 to 20°C) (Tinc treatment) or to a lower temperature (from 20 to 10°C) (Tdec treatment) for 72h. At 1, 3, 12, 24, 72h of exposure, animals were removed and prepared for further analysis. Results showed
that the effect of acute temperature changes on enzyme activities was significant. In Tinc treatment, activities of SOD and CAT increased immediately. The significant enhancement of SOD and CAT activities suggested
that oxidative stress increases significantly when ambient temperature increasing from 10 to 20°C. The up-regulation of Hsp70
in Tinc and Tdec treatments indicated that Hsp70 was a bioindicator of thermal stress in the sea cucumber, and the expression pattern depended
on the thermal treatment. |
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| Keywords: | antioxidant enzyme Apostichopus japonicus hexokinase oxidative stress pyruvate kinase sea cucumber Hsp70 thermal stress |
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