10个菲律宾蛤仔野生群体的遗传多样性研究

对我国沿海地区10个菲律宾蛤仔野生群体线粒体16S r RNA和COI基因部分序列进行测序,分别得到了长度为473bp和632bp的片段。结果表明,16S r RNA 193条序列A+T平均含量为66.6%,共检测到21个变异位点,193个个体具有22种单倍型;COI基因183条序列A+T平均含量为64.8%,共检测到126个变异位点,183个个体具有67种单倍型。基于群体间遗传距离利用Mega5.1软件构建10个群体的NJ树,聚类结果表明,大连群体和荣成群体聚为一支,其余8个群体聚为一支。AMOVA分析表明,大连群体和荣成群体间分化不显著,而荣成、大连群体与其余8个群体间的分化达到极显著水平(P0.01),说明我国沿海的菲律宾蛤仔野生群体存在一定的遗传分化。

THE GENETIC DIVERSITY OF TEN WILD POPULATIONS OF RUDITAPES PHILIPPINARUM

Partial sequences of the mitochondrial 16S rRNA gene and COI gene of 10 Ruditapes philippinarum populations in China were sequenced, from which 473bp from 16S rRNA gene and 632bp from COI gene were obtained. The average contents of A+T (16S rRNA gene 66.6% and COI gene 64.8%) were significantly higher than those of G+C. In 16S rRNA gene fragment, 21 variation sites were detected and 22 haplotypes were defined. In COI gene fragment, 126 variation sites were detected and 67 haplotypes were defined. Based on the genetic distance among populations, the NJ phylogenetic trees were obtained. The trees display that Dalian and Rongcheng Populations are clustered in one clade, the rest 8 populations to another. AMOVA analysis showed that genetic differentiation between Dalian and Rongcheng Populations was not significant, while it is extremely significant among Dalian-Rongcheng Populations and the rest of eight populations(P<0.01), indicating certain genetic differentiation among different wild populations of R. philippinarum in China.