| 养殖卵形鲳鲹诺卡氏菌病的研究 |
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| 引用本文： | 王瑞旋,刘广锋,王江勇,冯娟,郭志勋,徐力文.养殖卵形鲳鲹诺卡氏菌病的研究[J].海洋湖沼通报,2010(1). |
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| 作者姓名： | 王瑞旋 刘广锋 王江勇 冯娟 郭志勋 徐力文 |
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| 作者单位： | 中国水产科学研究院南海水产研究所,广东,广州,510300 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项资金项目(2009TS06)资助 |
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| 摘 要： | 报道了卵形鲳鲹(Trachinotus ovatus)诺卡氏菌病的发生情况。病鱼以体表和心、脾、肾等内脏出现白色结节为主要症状。对病鱼进行细菌分离培养和光镜、电镜检查,均发现长或短的丝状分枝状杆菌,用分离得到的菌株进行回归感染,证实为此次卵形鲳鲹结节病的病原菌。对病原菌的基本生物学特性进行了研究,并结合分子生物学方法进行鉴定,确定病原为鰤鱼诺卡氏菌(No-cardia seriolae),同时对病原菌进行药物敏感检测,发现该病原菌对多种常用水产药物均产生了抗药性,纸片扩散法结果显示仅20种受试药物中仅对环丙沙星、氯霉素、链霉素等中度敏感,试管稀释法结果显示8种药物中仅对1×10-6的盐酸恩诺沙星和盐酸环丙沙星敏感。
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| 关 键 词： | 卵形鲳鲹 结节病 鰤鱼诺卡氏菌 |
STUDIES ON NOCARDIASIS INFECTED FARMING TRACHINOTUS OVATUS |
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| WANG Ruixuan,LIU Guangfeng,WANG Jiangyong,FENG Juan,GUO Zhixun,XU Liwen.STUDIES ON NOCARDIASIS INFECTED FARMING TRACHINOTUS OVATUS[J].Transaction of Oceanology and Limnology,2010(1). |
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| Authors: | WANG Ruixuan LIU Guangfeng WANG Jiangyong FENG Juan GUO Zhixun XU Liwen |
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| Institution: | South China Sea Fisheries Research Institute;Chinese Academyof Fisheries Sciences;Guangzhou 510300;China |
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| Abstract: | An epizootic in seawater cage-reared Trachinotus ovatus in China was reported in this paper.White nodules were scattered on the heart,spleen and kidne of diseased fish.The morphology of isolated bacteria from Lowenstein-Jensen medium(LJM) was Gram-positive branching,bead-like or long,slender,filamentous rods.Experimental infection indicated that the isolated bacterium was pathogen responsible for mortalities.Based on the symptom of diseased fish and the growth characteristics,morphological,physiological,and... |
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| Keywords: | Trachinotus ovatus sarcoidosis Nocardia seriolae |
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