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Molecular diversity of Enteromorpha from the coast of Yantai: a dual-marker assessment |
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| Authors: | Haiyan Liu Zhengyi Liu Yinchu Wang Yushan Zhao Song Qin |
| Institution: | 1. University of Chinese Academy of Sciences, Beijing, 100049, China 2. Yantai Institute of Coastal Zone, Chinese Academy of Sciences, Yantai, 264003, China 3. Key Lab of Marine Biology in Jiangsu, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, China 4. Shandong Oriental Ocean Sci-Tech Co. Ltd., Yantai, 264003, China |
| Abstract: | We collected nine Enteromorpha specimens from the coast of Yantai and evaluated their diversity based on analyses of their ITS (internal transcribed spacer) and 5S rDNA NTS (non-transcribed spacer) sequences. The ITS sequences showed slight nucleotide divergences between Enteromorpha linza and Enteromorpha prolifera. In contrast, multiple highly variable regions were found in the ITS region of Enteromorpha flexuosa. In general, there were more variable sites in the NTS region than in the ITS region in the three species. The variations in 5S rDNA NTS sequences indicated that the molecular diversity of Enteromorpha from the coast of Yantai is very high. However, a phylogenetic tree constructed using 5S rDNA NTS sequence data indicated that genetic differences were not directly related to geographical distribution. |
| Keywords: | Enteromorpha internal transcribed spacer （ITS） 5SrDNA non-transcribed spacer （NTS） molecular diversity |
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