镉、铜对非洲鲫鱼肝线粒体功能影响的初步研究 |
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引用本文: | 刘克强.镉、铜对非洲鲫鱼肝线粒体功能影响的初步研究[J].海洋科学,1984,8(6):29-31. |
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作者姓名: | 刘克强 |
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作者单位: | 中国科学院海洋研究所 |
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摘 要: | 本实验以在海水中培养的非洲鲫鱼(Tilapiamossambica)为试验材料,采用氧电极法测定氯化镉、硫酸铜对鱼肝线粒体(离体)呼吸控制率(RCR)和氧化磷酸化效率(ADP/O)的影响,以探讨重金属毒物对海洋生物的致毒机理以及了解对其体內各种生化过程所起的作用。
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THE EFFECT OF CADIUM CHLORIDE AND COPPER SULFATE ON THE FUNCTION OF LIVER MITOCHONDRIA OF TILAPIA MOSSAMBICA |
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Abstract: | Cadium chloride and copper sulfate were used separately at different concentrations as toxic agent on the liver mitochondria of Tilapia mossambica in vitro. The degree of couplingof oxidative phosphorylation-respiratory control and ADP/O ratios of the liver mitochondria were examined by oxygen electrode measurements. In cases where sodium succinate was used as substrate, owing to inhibition of 1 ppm cadiumchloride and 5 ppm copper sulfate on liver mitochondria, RCR and ADP/O ratio were decreased obviously. |
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