Isolation and identification of alginate-degrading bacteria and formation of alginase

Five strains of alginate-degrading bacteria Were isolated from the decaying parts of Laminria japonicaand Undaria pinnatifida and identified as Alteromonas espejiana (Strain A101, A102, A103, A105 ) and Alteromonas macleodii(Strain A104). When incubated at 25 t for 144 h in the liquid mediuxn which contamed 0. 5%peptone, 0. 3% -0. 6%sodium alginate, 0. 1 % yeast extract, 3% NaCI, pH 7. 5, the stain A102 pnduced the highest amount of alginase.