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泥蚶(Tegillarca granosa)遗传连锁图谱的构建
引用本文:刘 博,滕爽爽,柴雪良,邵艳卿,肖国强,张炯明,方 军.泥蚶(Tegillarca granosa)遗传连锁图谱的构建[J].海洋与湖沼,2012,43(5):983-991.
作者姓名:刘 博  滕爽爽  柴雪良  邵艳卿  肖国强  张炯明  方 军
作者单位:浙江省海洋水产养殖研究所,温州325000 浙江省近岸水域生物资源开发与保护重点实验室,温州325000
基金项目:国家高技术研究发展计划(863 计划), 2012AA10A410-1 号; 浙江省自然科学基金项目, Y3110003; 浙江省科研院所专项, 2009F20010号; 温州市科技兴海项目, S20080019 号
摘    要:采用AFLP标记技术结合"拟测交"策略,对泥蚶父、母本和109个F1全同胞个体进行了遗传连锁的分析研究,构建了泥蚶的遗传连锁图谱。结果表明,在638个分离标记中,符合1:1孟德尔分离类型位点的母本和父本分离标记分别为235和259个;3:1孟德尔分离类型位点59个。雌性图谱包括21个连锁群,标记间平均间隔为8.12cM,图谱共覆盖1737.8cM,覆盖率为83.1%;雄性图谱包括20个连锁群,标记间平均间隔为5.81cM,图谱共覆盖1388.2cM,覆盖率为84.7%。

关 键 词:泥蚶  AFLP  遗传连锁图谱  拟测交策略
修稿时间:2011/12/19 0:00:00

CONSTRUCTION OF GENETIC LINKAGE MAP OF TEGILLARCA GRANOSA LINNAEUS
LIU Bo,TENG Shuang-Shuang,CHAI Xue-Liang,SHAO Yan-Qing,XIAO Guo-Qiang,ZHANG Jiong-Ming and FANG Jun.CONSTRUCTION OF GENETIC LINKAGE MAP OF TEGILLARCA GRANOSA LINNAEUS[J].Oceanologia Et Limnologia Sinica,2012,43(5):983-991.
Authors:LIU Bo  TENG Shuang-Shuang  CHAI Xue-Liang  SHAO Yan-Qing  XIAO Guo-Qiang  ZHANG Jiong-Ming and FANG Jun
Institution:Zhejiang Mariculture Research Institute; Zhejiang Key Laboratory of Exploitation and Reservation of Coastal Bio-Resource;Zhejiang Mariculture Research Institute; Zhejiang Key Laboratory of Exploitation and Reservation of Coastal Bio-Resource;Zhejiang Mariculture Research Institute; Zhejiang Key Laboratory of Exploitation and Reservation of Coastal Bio-Resource;Zhejiang Mariculture Research Institute; Zhejiang Key Laboratory of Exploitation and Reservation of Coastal Bio-Resource;Zhejiang Mariculture Research Institute; Zhejiang Key Laboratory of Exploitation and Reservation of Coastal Bio-Resource;Zhejiang Mariculture Research Institute; Zhejiang Key Laboratory of Exploitation and Reservation of Coastal Bio-Resource;Zhejiang Mariculture Research Institute; Zhejiang Key Laboratory of Exploitation and Reservation of Coastal Bio-Resource
Abstract:
Keywords:Tegillarca granosa  AFLP  Genetic linkage map  Double pseudo-testcross strategy
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