山东沿海21种海洋无脊椎动物抗肿瘤活性初步筛选

采用四氮唑盐比色法(MTT)测定了21种青岛及其附近海域常见无脊椎动物94％乙醇粗提物对人白血病细胞HL-60和人肺腺癌细胞A-549的抑制率，同时测定了海筒螅Tubuaria marinha Torrey等动物不同极性溶剂萃取物的活性。发现海燕Asterina pectinifera的正丁醇萃取物、海筒螅的乙酸乙酯萃取物、红条毛肤石鳖Acanthochiton rubrolineatus的94％的乙醇提取物对HL-60的抑制作用很强，在浓度为0．063mg／mL时抑制率分别为96．7％、63．9％、50．5％；海筒螅的乙酸乙酯萃取物对A．549的抑制率较高，浓度为0．063mg／mL时的抑制率可以达到65.4%.

ANTICANCER ACTIVITY OF 21 MARINE INVERTEBRATE SPECIES

The anticancer activity of 21 marine invertebrate species which are common near Qingdao seashore was determined by MIT method. It was found that the n-butanol part of Asterina pectinifera, the acetic ether part of Tubuaria marina Torrey, 94% ethanol extract of Acanthochiton rubrolineatus have much stronger inhibition rates on tumor cell HL60, which are 96. 7%, 63.9% and 50. 5% respectively in the concentration of 0.063mg/mL. The inhibition rate of the acetic ether part of Tubuaria marina Torrey on the tumor cell A-549 is 65. 4 % in the concentration of 0. 063 mg/mL.