急性Pb2+胁迫对大口黑鲈(Micropterus salmoides)幼鱼Hippo信号通路基因表达的影响

为研究重金属铅离子对大口黑鲈(Micropterus salmoides)幼鱼Hippo信号通路中主要基因表达的影响,本实验采用qPCR技术研究了96 h急性不同浓度铅胁迫(0、10、17.8、31.6、56.2和100 mg/L)下Hippo信号通路中的部分基因在肝脏、肌肉、鳃和小肠组织中的mRNA表达量变化。结果显示:与对照组(0 mg/L)相比,七个基因在肝脏组织中表达量变化总体上呈上升趋势,除Lats1/2外,其他基因在铅胁迫浓度(17.8 mg/L)时都显著上调(P<0.05);在肌肉组织中,MOB1表达量在不同浓度铅胁迫下上升显著(P<0.05);在腮组织中,YAP/TAZ、TEAD、PP2A、MOB1、KIBRA和FRMD表达量在铅胁迫浓度(10 mg/L)时显著上调(P<0.05);在小肠组织中,PP2A、KIBRA和14-3-3表达量显著下降(P<0.05)。结果提示大口黑鲈可能通过调节Hippo信号通路中相关基因的表达响应铅胁迫。

EFFECT OF ACUTE Pb2+ STRESS ON THE EXPRESSION OF GENES INVOLVED IN HIPPO SIGNALING PATHWAY IN JUVENILE LARGEMOUTH BASS MICROPTERUS SALMOIDES

To study the genes in Hippo signaling pathway of juvenile largemouth bass Micropterus salmoides, the mRNA expressions of genes that involved in Hippo signaling pathway in the liver, muscle, gill and intestine of the fish species undergone acute Pb²⁺ stress in different concentrations (0, 10, 17.8, 31.6, 56.2, and 100 mg/L) for 96 h was investigated, and the genes included KIBRA, FRMD, Lats1/2, MOB1, PP2A, YAP/TAZ, 14-3-3, and TEAD. Results show that in the tissue of liver, the mRNA expression levels of these genes were increased compared to the control group, and the genes expressions were up-regulated significantly at Pb²⁺ concentration of 17.8 mg/L in 96 h, except for Lats1/2. In the muscle, the expression of MOB1 was increased significantly at all the different Pb²⁺ concentrations. In the gill, the mRNA expressions of genes including YAP/TAZ, TEAD, PP2A, MOB1, KIBRA, and FRMD up-regulated dramatically at 10 mg/L in 96 h. In the tissue of intestine, PP2A, KIBRA, and 14-3-3 expressions were significantly down-regulated in this study. Therefore, the regulation of mRNA expression of related genes in Hippo signaling pathway had a molecular mechanism to respond against acute Pb²⁺ stress in largemouth bass.