| Design and application of the method for isolating magnetotactic bacteria |
| |
| 引用本文： | XIAO Zhijie LIAN Bin CHEN Jun H. Henry Teng. Design and application of the method for isolating magnetotactic bacteria[J]. 中国地球化学学报, 2007, 26(3): 252-258. DOI: 10.1007/s11631-007-0252-y |
| |
| 作者姓名： | XIAO Zhijie LIAN Bin CHEN Jun H. Henry Teng |
| |
| 作者单位： | Department of |
| |
| 基金项目： | Foundation supported research project： Jiangsu Postdoctoral Science Foundation （No. 05010liB） and The Funds for Attracting Talents of Nanjing Normal University （184070H2B39）. |
| |
| 摘 要： | A simple apparatus was designed to effectively isolate magnetotactic bacteria from soils or sediments based on their magnetotaxis. Through a series of processes including sample incubation, MTB harvesting, isolation, purification and identification, several strains of bacteria were isolated from the samples successfully. By Transmission Electron Microscopy (TEM) and Energy-Dispersive X-ray Analysis (EDXA), these bacteria were certificated to be magnetotactic bacteria. The phylogenetic relationship between the isolated magnetic strains and some known magnetotactic bacteria was inferred by the construction of phylogenetic tree based on 16SrDNA sequences. This apparatus has been proven to have the advantages of being inexpensive, simple to assemble, easy to perform and highly efficient to isolate novel magnetotactic bacteria. The research indicated that the combined approach of harvesting MTB by home-made apparatus and the method of plate colony isolation could purify and isolate magnetotactic bacteria effectively.
|
| 关 键 词： | 趋磁性 隔离带 器械 化学成分 |
| 收稿时间： | 2006-06-01 |
| 修稿时间： | 2006-06-012006-07-03 |
Design and application of the method for isolating magnetotactic bacteria |
| |
| Xiao Zhijie,Lian Bin,Chen Jun,H. Henry Teng. Design and application of the method for isolating magnetotactic bacteria[J]. Chinese Journal of Geochemistry, 2007, 26(3): 252-258. DOI: 10.1007/s11631-007-0252-y |
| |
| Authors: | Xiao Zhijie Lian Bin Chen Jun H. Henry Teng |
| |
| Affiliation: | (1) Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Biological Sciences, Nanjing Normal University, Nanjing, 210097, China;(2) Institute of Surficial Geochemistry, Department of Earth Sciences, Nanjing University, Nanjing, 210093, China;(3) Department of Chemistry, the George Washington University, Washington, DC, 20052, USA |
| |
| Abstract: | A simple apparatus was designed to effectively isolate magnetotactic bacteria from soils or sediments based on their magnetotaxis. Through a series of processes including sample incubation, MTB harvesting, isolation, purification and identification, several strains of bacteria were isolated from the samples successfully. By Transmission Electron Microscopy (TEM) and Energy-Dispersive X-ray Analysis (EDXA), these bacteria were certificated to be magnetotactic bacteria. The phylogenetic relationship between the isolated magnetic strains and some known magnetotactic bacteria was inferred by the construction of phylogenetic tree based on 16SrDNA sequences. This apparatus has been proven to have the advantages of being inexpensive, simple to assemble, easy to perform and highly efficient to isolate novel magnetotactic bacteria. The research indicated that the combined approach of harvesting MTB by home-made apparatus and the method of plate colony isolation could purify and isolate magnetotactic bacteria effectively. |
| |
| Keywords: | magnetotactic bacteria home-made apparatus isolation identification |
| 本文献已被 CNKI 维普 SpringerLink 等数据库收录！ |
|
