中国南海短指软珊瑚中化学成分的研究

运用硅胶柱层析、Sephadex LH-20凝胶柱层析和HPLC等技术,采用活性追踪分离的方法,对南海短指软珊瑚Sinularia sp.的化学成分进行了研究.从乙醇提取物中分离获得单体化合物,运用NMR,MS等波谱技术鉴定了8个化合物的结构,分别为:3β-羟基-24-亚甲基胆甾-5-烯-7-酮(1),孕甾烯醇酮(2),cembrene A(3),epoxycembrane A(4),4,8,12-trimethyl-1-(1-methylethenyl)-3,7-cyclotetradecadien-10-one(5),(3E,7E,11E)-11,12-dihydroxy-1-isopropyl-4,8,12-trimethyicyclotetradeca-1,3,7-triene(6),邻苯二甲酸二丁酯(7)和十九烷-2-酮(8).化合物5和8为首次从珊瑚动物中分离得到,6为首次从短指软珊瑚属中分离得到.化合物3和4显示较强的卤虫致死活性.西松烷二萜类化合物是该短指软珊瑚中的重要活性成分.

Studies on the Chemical Constituents of the Soft Coral Sinularia sp. from the South China Sea

The chemical constituents of soft coral Sinularia sp. from the South China Sea were investigated by bioassay-guided fractionation. The ethanolic extract was separated by repeated column chromatography on silica gel and Sephadex LH-20 and HPLC to obtain eight compounds. Their structures were elucidated as 3β-hydroxy-24-methylenecholest-5-en-7-one(1), pregnenolone(2), cembrene A(3), epoxycembrane A(4), 4, 8, 12-trimethyl-1-(1-methylethenyl)-3, 7-cyclotetradecadien-10-one(5), (3E,7E,11E)-11,12-dihydroxy-1-isopropyl-4, 8,12-trimethyicyclotetradeca-1, 3, 7-triene(6), 1, 2-benzenedicarboxylic acid, isobutyl ester(7) and 2-nonadecanone(8), respectively, on the basis of their spectroscopic data including NMR and MS. Compounds 5 and 8 were isolated from corals for the first time, and compound 6 was firstly isolated from the genus of Sinularia. Compounds 3 and 4 exhibited high lethal rates to brine shrimp Artemia salina. Cembrane-type diterpenoids were deduced as important bioactive compounds in the soft coral Sinularia sp.