Microsatellite DNA polymorphisms and the relation with body weight in sea cucumber <Emphasis Type="Italic">Apostichopus japonicus</Emphasis> |
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Authors: | Xiuli Wang Xue Shan Xuemei Qiu Xiangying Meng Yaqing Chang |
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Institution: | (1) Key Laboratory of Mariculture and Biotechnology of Agriculture Ministry, Dalian Fisheries University, Dalian, 116023, China;(2) College of Life Sciences and Biotechnology, Dalian Fisheries University, Dalian, 116023, China |
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Abstract: | The relationship between microsatellite polymorphism and body weight of captive bred Chinese sea cucumber Apostichopus japonicus was investigated in two local populations in Dalian. Among ten loci discovered, nine show changes except for AJ07 loci. Seven
loci were found highly polymorphic in both populations. For each locus in two populations, the average number of alleles is
6.428 6 and 6.285 7, the average observed heterozygosity at 0.225 7 and 0.245 9, the expected heterozygosity at 0.776 8 and
0.748 8, the polymorphism information content (PIC) at 0.709 2 and 0.674 6, respectively. Further analysis show significant
correlation between A. japonicus body weight and occurrence markers AJ02 and AJ04. The findings of the relation may be helpful for molecular breeding, as
well as the marker-assisted selection of sea cucumbers.
Supported by the National High Technology Research and Development Program of China (863 Program, No. 2006AA10A411), the Natural
Science Foundation of Liaoning Province (No.20072139), the Grant of Dalian Fisheries University, the Key Laboratory Foundation
of the Educational Department of Liaoning Province (No.2009S024) |
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Keywords: | Keyword" target="_blank">Keyword microsatellite DNA polymorphism correlation analysis body weight Apostichopus japonicus |
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