氨氮对菲律宾蛤仔免疫力的影响

通过氨氮对菲律宾蛤仔(Ruditapes philippinarum)的急性毒性实验,发现氨氮质量浓度对数与死亡概率单位之间的相关性方程为y=1.827 1x 0.651 9,R2=0.971 9,据此方程计算出,氨氮对菲律宾蛤仔的24 h半致死浓度为239.88 mg/L,24 h内引起5%个体死亡的氨氮质量浓度为13.49 mg/L。通过氨氮对菲律宾蛤仔血细胞数量和血淋巴溶菌活力的影响实验,发现在各取样时间下,菲律宾蛤仔血细胞数量随着氨氮质量浓度的增加而降低,且差异显著(F﹥F0.05);随着时间的延长,各个处理组中菲律宾蛤仔的血细胞数量表现出相同的变化趋势,即在受到氨氮胁迫后6 h时达到最低值,之后有所升高,但第12小时与第24小时菲律宾蛤仔血细胞数量无显著差异(F﹤F0.05)。氨氮对菲律宾蛤仔血淋巴溶菌活力影响显著(F﹥F0.05),各取样时间下,随着氨氮质量浓度的升高,其血淋巴溶菌活力逐渐降低;第6小时,各氨氮质量浓度下的溶菌活力均达到最低值,同一氨氮浓度下第6,12,24小时时的溶菌活力无显著差异(F﹤F0.05)。

The effect of ammonia-N on immune activity of Ruditapes philippinarum

By the acute toxic experiment on Ruditapes philippinarum of ammonia-N,the related equation between the concentration of ammonia-N and the mortality of R.philippinarum was y =1.827 1x 0.651 9,R2=0.971 9.According to this equation,it was calculated that the half death in 24 h of R.philippinarum was about 239.88 mg/L,the concentration of ammonia-N which caused 5% death in 24h was 13.49 mg/L.By the experiment on the influence of ammonia-N on the number of haemocyte and the bacteriolytic activity,it was found that the number of haemocytes of R.philippinarum decreased as increasing the concentration of ammonia-N in different times,and their difference was remarkable(F>F0.05).As the time increasing,there was the same change trend of the number of haemocyte of R.philippinarum.It reached minimum when reacted for 6h,after which it became increased.The number of haemocyte had no significant difference in the 12h and 24h(F>F0.05) reaction times.