About the Role of Quinones in the Action of Some Polyphenols on the Streaming of Protoplasm in Nitella sp. Cells

The concentration of phenols and quinones which stopped the protoplasmic movement in the Nitella cells after 15 min was determined. The quinones (β-naphthoquinone, α-naphthoquinone, p-benzoquinone, o-toluquinone, o-bromanil) stopped the protoplasmic movement in the concentration of (1 3) 10^?4 M, phenols (m-cresol, p-cresol, hydroquinone, guaiacol, phenol, gallic acid, pyrogallic acid, resorcinol) in the concentration of (0.2 5) 10^?2 M, except catechol – 2 10^?3 M. Very high toxicity of catechol and in the prolonged experiments (3 hours) also of hydroquinone is connected with the action of quinonoid productions of its oxidation. This suggestion is confirmed by the experiments:

• – by adding the phenol solutions to Nitella shoots (catechol, hydroquinone, resorcinol) we observed the greatest increase of oxygen absorption in case of catechol (86%), less in case of hydroquinone (38%), and for resorcinol oxygen absorption practically remained on the control level;
• – the action of catechol, hydroquinone and p-benzoquinone did not take place in the presence of glutathione and cysteine (but not cystine and oxidated glutathione);
• – diethyldithiocarbamate as an inhibitor of o-diphenoloxidase reduced the ability of catechol solutions to stop the protoplasmic movement;
• – the low concentrations of benzoquinones were able to stop the protoplasmic movement;
• – for stopping the protoplasmic movement under the prolonged time of incubation from 15 min to 3 hours there was necessary a lower concentration of solutions of hydroquinone and catechol in comparison with resorcinol.