| Construction of Porphyra yezoensis Pure Line from Protoplasts and Its 18S rDNA Sequence Determination |
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| 作者姓名： | LIUHongquan YUWengong DAIJixun GONGQianhong SHIXiaochong YANGKunfeng |
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| 作者单位： | [1]InstituteofMarineDrugsandFoods,OceanUniversityofChina,Qingdao266003,P.R.China [2]CollegeofMarineLifeSciences,OceanUniversityofChina,Qingdao266003,P.R.China |
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| 摘 要： | The wild Porphyra yezoensis collected from the Qingdao coast was used to prepare protoplasts by enzyme digestion. The pure line was constructed by cultivating the protoplasts. The 18S rDNA of the P. yezoensis pure line was cloned and sequenced. Sequence analysis was executed for this sequence and other 22 sequences retrieved from GenBank. A phylogenetic tree was constructed using the neighbor-joining method. The results revealed a high diversity of 18S rDNA sequences in genus Porphyra and the considerable variation of 18S rDNA sequences in different strains of the same species P. yezoensis and P. tenera. Significant difference of 18S rDNA sequence was observed between P. yezoensis from Qingdao, China, and the two strains of P. yezoensis from Japan, but the three strains of P. yezoensis formed a stable clade in the phylogenetic tree. These results indicate the possibility of interspecies and intraspecies discrimination of Porphyra using the 18S rDNA sequences.
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| 关 键 词： | DNA序列分析 种间区别 种内区别 海洋藻类 |
| 收稿时间： | 27 November 2003 |
| 修稿时间： | 2 February 2004 |
Construction of <Emphasis Type="Italic">Porphyra yezoensis</Emphasis> pure line from protoplasts and its 18S rDNA sequence determination |
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| LIUHongquan YUWengong DAIJixun GONGQianhong SHIXiaochong YANGKunfeng.Construction of Porphyra yezoensis Pure Line from Protoplasts and Its 18S rDNA Sequence Determination[J].Journal of Ocean University of China,2004,3(1):60-64. |
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| Authors: | Liu Hongquan Yu Wengong Dai Jixun Gong Qianhong Shi Xiaochong Yang Kunfeng |
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| Institution: | (1) Institute of Marine Drugs and Foods, Ocean University of China, 266003 Qingdao, P.R.China;(2) College of Marine Life Sciences, Ocean University of China, 266003 Qingdao, P.R.China |
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| Abstract: | The wild Porphyra yezoensis collected from the Qingdao coast was used to prepare protoplasts by enzyme digestion. The pure line was constructed by cultivating
the protoplasts. The 18S rDNA of the P. yezoensis pure line was cloned and sequenced. Sequence analysis was executed for this sequence and other 22 sequences retrieved from
GenBank. A phylogenetic tree was constructed using the neighbor-joining method. The results revealed a high diversity of 18S
rDNA sequences in genus Porphyra and the considerable variation of 18S rDNA sequences in different strains of the same species P. yezoensis and P. tenera. Significant difference of 18S rDNA sequence was observed between P. yezoensis from Qingdao, China, and the two strains of P. yezoensis from Japan, but the three strains of P. yezoensis formed a stable clade in the phylogenetic tree. These results indicate the possibility of interspecies and intraspecies discrimination
of Porphyra using the 18S rDNA sequences. |
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| Keywords: | Porphyra yezoensis 18S rDNA sequence analysis phylogenetic tree |
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