Genetics and phylogeny of genus Coilia in China based on AFLP markers |
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| Authors: | Qiaoli Yang Zhiqiang Han Dianrong Sun Songguang Xie Longshan Lin Tianxiang Gao |
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| Institution: | (1) Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing, 210017, China;(2) College of Life Science, Wuhan University, Wuhan, 430072, China;(3) Jiangsu Engineering Research Center for Wild Aquatic Animals Introduction & Hatching, Nanjing, 210017, China;(4) Center of Material Analysis, Nanjing University, Nanjing, 210093, China;(5) School of Biological Science & Medical Engineering, Southeast University, Nanjing, 210096, China;(6) Animal, Plant Quarantine and Food Inspection Center of Jiangsu Exit and Entry Inspection and Quarantine Bureau, Nanjing, 210001, China;(7) Department of Veterinary Biomedical Sciences and Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan, Canada;(8) Zoology Department, Center for Integrative Toxicology, Michigan State University, East Lansing, MI 48824, USA;(9) Centre for Coastal Pollution and Conservation, Department of Biology and Chemistry, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong SAR, China;(10) Environmental Science Program, Nanjing University, Nanjing, 210093, China; |
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| Abstract: | The taxonomy of Coilia has been extensively studied in China, and yet phylogenetic relationships among component taxa remain controversial. We used
a PCR-based fingerprinting technique, amplified fragment length polymorphism (AFLP) to characterize and identify all four
species of Coilia in China. We examined the genetic relationships of the four species of Coilia and a subspecies of Coilia nasus with AFLP. A total of 180 AFLP loci were generated from six primer combinations, of which 76.11% were polymorphic. The mean
genetic distance between pairs of taxa ranged from 0.047 to 0.596. The neighbor-joining tree and UPGMA dendrogram resolved
the investigated species into three separate lineages: (1) C. mystus, (2) C. grayii and (3) C. brachygnathus, C. nasus, and C. nasus taihuensis. Phylogenetic analysis of the AFLP data is inconsistent with current morphological taxonomic systems. The AFLP data indicated
a close relationship among C. brachygnathus, C. nasus taihuensis, and C. nasus. Therefore, the two species described under Coilia (C. brachygnathus and C. nasus taihuensis) are treated as synonyms of C. nasus. |
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