栉孔扇贝(Chlamys (Azumapecten) farreri) 消化盲囊的组织学和组织化学的研究

运用石蜡切片法、透射电镜技术及组织化学方法，对栉孔扇贝的消化盲囊进行了研究。结果表明消化盲囊的腺上皮由消化(吸收)细胞和分泌细胞组成，消化(吸收)细胞顶端有微绒毛，细胞内有酸性磷酸酶、碱性磷酸酶、脂酶和酯酶活性，还含有糖原和脂肪。是进行食物的细胞内消化和营养物质吸收的主要场所，并有储存能量的功能；分泌细胞内含粗面内质网、高尔基体和分泌颗粒，具有酸性磷酸酶和碱性磷酸酶活性。并含有丰富的RNA和蛋白质，有分泌消化酶的功能；消化盲囊导管上皮细胞顶端有纤毛和微绒毛，细胞内有酸性磷酸酶、碱性磷酸酶及脂酶活性，也能分泌消化酶。消化(吸收)细胞浅部的细胞质及其释放到盲囊腔中的胞质团中含铁；各部位不含钙。

A Histological and Histochemical Study on Digestive Diverticula of Farreris's Scallop, Chlamys (Azumapecten) farreri

The structural and functional aspects of the digestive diverticula of Chlamys farreri were studied using light and transmission electron microscopy and histochemical methods. The epithelium of the digestive diverticula contains two types of cells, namely the digestive (absorptive) and secretory cells. The digestive cell is lined with microvilli, the presence of acid phosphatase activity in digestive diverticula suggest that these cells are actively involved in the absorption and intracellular digestion of food material, and the absorption role is supported by the presence of alkaline phosphatase in the digestive diverticula. Esterase and lipase are detected in the digestive cell. Lipid and glycogen are abundant throughout the digestive cell, showing that these cells are the sites of energy stores. The secretory cells contain abundant protein and RNA suggesting that they have synthetic function, together with their cytological feathers: numerous rough endoplasmic reticulum, Golgi complex and secretorylike granules support the view that in Farreri's scallop Chlamys (Azumapecten) farreri the secretory cell plays an important role in the production and release of digestive enzyme. The duct of the digestive diverticula is lined with cilia and microvilli, acid and alkaline phosphatase as well as lipase activity are detected in the duct cells. Therefore, it can produce digestive enzyme. Fe is present in the distal parts and the fragmentation sphere of the digestive cell, and no calcium exists in the digestive diverticula.