褐煤中天然产甲烷菌富集培养与生物气产出模拟

采用厌氧培养方法，在云南省昭通褐煤样品中成功地培养富集了活性厌氧细菌，并进一步开展了为期60天的生物气生成模拟实验，分析了生物气的生成规律、物质组成和成因机制。结果揭示：3件褐煤样品中均有活性厌氧细菌存在，以纤维素分解菌为主，活性硫酸盐还原菌极其微少，说明昭通盆地褐煤层具有较强还原性的生化环境，有利于产甲烷菌的繁盛；生物气的生成经历了生气量缓慢增长、显著增高、趋于减缓三个阶段，表明产甲烷菌在经历了第一阶段缓慢繁殖后，其数量和活性在第二阶段达到较高水平；3件煤样生气量和生气历程存在一定差别的原因，可能在于原煤中活性产甲烷菌数量以及显微组分组成方面存在差异，褐煤生物气生成过程至少存在两个以上的生气高峰阶段。模拟实验中产生的生物气几乎全部由CH4和CO2组成，且CH4占主要部分，几乎没有检测到重烃气。甲烷气体δ13C1和δD的平均值均处于生物甲烷碳、氢同位素组成的正常分布范围。

Enrichment and Cultivation of Natural Methanogen and Simulation of Biogenetic Gas Generation from Brown Coal Samples

Using the method of anaerobic glove chamber culture, active anaerobic bacteria were successfully enriched and cultivated in brown coal samples collected from Zhaotong Basin, Yunnan Province. Simulation experiments of biogenic gas for 60 days were further conducted to analyze the generation regularities, substance compositions and genetic mechanism of biogenic gas. The results showed that in all three brown coal samples there existed active anaerobic bacteria, among which cellulose decomposition bacteria wer...