纤粒矿物粉尘体外细胞毒性研究

采用体外细胞培养技术，观察兔肺泡巨噬细胞死亡率，用丙二醛（ＭＤＡ）的含量变化、乳酸脱氢酶（ＬＤＨ）及超氧化物歧化酶（ＳＯＤ）的活性变化来评价来自１２个矿６床的６种矿物的１２种结晶习性的矿物粉尘的细胞毒性，探讨其使巨噬细胞受损的机制。结果显示：沸石、硅灰石无细胞毒性，而其他的纤维状及片粒状矿物粉尘则表现出不同程度的细胞毒性，说明矿物粉尘的细胞毒性与矿物粉尘的形态有一定关系，但主要由矿物粉尘的特性所决

VITRO Cytotoxicity Investigation of Fibrous and Grainy Mineral Dusts

In order to study the damaging mechanism of pulmonary alveolar macrophages, the changes of their death ratio,malandialdthyde (MDA) content and activities of dehydrogenate (LDH) and superoxide dismitase (SOD) were determined, and the technique of VITRO cell culture was used to investigate the cytotoxicity of dusts of six minerals (twelve cryst habits) from twelve mineral deposits. The results show that wollastonite and zeolite have no cytotoxicity while dusts of other fibrous and grainy minerals may damage pulmonary alveolar macrophages in various degrees. The cytotoxicity of fibrous mineral dust exceeds that of the grainy one; the cytotoxicity of dust is positively correlated with the active OH - content in dust, but not necessarily so with its SiO 2 content; the high pH values produced by dust is unfavorable for the survival of cells; the dust with a low bio_resistance is safe for cells; the content of variable_valency elements may influence cytotoxicity. It is suggested that the shape of mineral dusts is one of the factors affecting cytotoxicity, and that the cytotoxicity of mineral dusts mainly depends on its properties.