| Changes of proteins in the Antarctic ice microalga Chlamydomonas sp.cultured under UV-B radiation stress |
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| 作者姓名： | KAN Guangfeng MIAO Jinlai SHI Cuijuan LI Guangyou |
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| 作者单位： | KAN Guangfeng1，2*，MIAO Jinlai2，SHI Cuijuan1，LI Guangyou21. School of Ocean，Harbin Institute of Technology in Weihai，Weihai 264209，China2. State Oceanic Administration Key Laboratory of Marine Bioactive Substances，Qingdao 266061，ChinaReceived 8 December 2005; accepted 1 February 2006 |
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| 摘 要： | 1 IntroductionAntarctic ice m icroalga Chlamydomonas sp., akind ofgreen algae in the Antarctic sea ice, playsavery important role in Antarctic ecological environm ents.Antarctic“ozonehole”enhancesthesolarUVradiationarrivingatseaiceandcontinent. M anyphy…
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| 关 键 词： | 海藻 适应装置 南极 蛋白质 |
| 收稿时间： | 2005-12-08 |
| 修稿时间： | 2006-02-01 |
Changes of proteins in the Antarctic ice microalga Chlamydomonas sp.cultured under UV-B radiation stress |
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| KAN Guangfeng,MIAO Jinlai,SHI Cuijuan,LI Guangyou.Changes of proteins in the Antarctic ice microalga Chlamydomonas sp.cultured under UV-B radiation stress[J].Acta Oceanologica Sinica,2006,25(2):135-141. |
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| Authors: | KAN Guangfeng MIAO Jinlai SHI Cuijuan and LI Guangyou |
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| Institution: | 1.School of Ocean, Harbin Institute of Technology in Weihai, Weihai 264209, China;State Oceanic Administration Key Laboratory of Marine Bioactive Substances, Qingdao 266061, China2.State Oceanic Administration Key Laboratory of Marine Bioactive Substances, Qingdao 266061, China3.School of Ocean, Harbin Institute of Technology in Weihai, Weihai 264209, China |
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| Abstract: | Antarctic ice microalga Chlamydomonas sp. can thrive undisturbed under high UV radiation in the Antarctic ice layer. However, it is unknown that the initial adaptation mechanisms in protein level occurring in response to high UV radiation. Global-expression profiling of proteins in response to stress was analyzed by two-dimensional electrophoresis (2-DE) and image analysis. In the 2-DE analysis, protein preparation is the key step. Three different protein extract methods were compared, and the results showed that the trichloroacetic acid (TCA)-acetone fractional precipitation method was the fittest one. At the same time, the proteins in Chlamydomonas sp. were compared in 2-DE way, and the synthesis of seven protein spots was found disappeared and 18 decreased after exposed to UV-B radiation. In addition, 14 protein spots were enhanced or induced, among which two new peptides (20 and 21 kDa) appeared whose isoelectric point (pI) was 7.05 and 4.60 respectively. These changed proteins might act as key role in the acclimation of Antarctic ice microalga to UV-B radiation |
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| Keywords: | Antarctic ice alga Chlamydomonas sp UV-B radiation 2-DE adaptation mechanisms |
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