| 日本(虫寻)和底栖短桨蟹线粒体DNA 12SrRNA基因序列的初步研究 |
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| 引用本文： | 高天翔,张秀梅,渡边精一,伏屋玲子.日本(虫寻)和底栖短桨蟹线粒体DNA 12SrRNA基因序列的初步研究[J].中国海洋大学学报(自然科学版),2004,34(1):43-47. |
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| 作者姓名： | 高天翔 张秀梅 渡边精一 伏屋玲子 |
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| 作者单位： | 1. 中国海洋大学教育部海水养殖重点实验室,山东,青岛,266003
2. 日本东京水产大学水生生物系,东京 108-8477 |
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| 基金项目： | 国家重点基础研究发展规划(G19990437);日本学术振兴会未来开拓学术研究项目(97L00902)资助 |
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| 摘 要： | 以相应引物对日本和底栖短桨蟹的线粒体 DNA1 2 S r RNA基因片段进行了 PCR扩增和序列测定 ,分析比较了 2种间序列差异。结果表明 :日本 1 2 S r RNA基因片段长度为 41 4 bp,底栖短桨蟹为 41 5bp,2种的 A,T,G,C含量分别为 1 51 bp(36.47% ) ,1 53bp(36.96% ) ,43bp(1 0 .39% ) ,67bp(1 6.1 8% )和 1 50 bp(36.1 4 % ) ,1 55bp(37.35% ) ,44bp(1 0 .60 % ) ,66bp(1 5.90 % )。 2种间共出现了 3个碱基的缺失 /插入和 38bp的序列差异 ,其碱基转换与碱基颠换比约为 2 .1 7。
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| 关 键 词： | 日本蟳 底栖短桨蟹 12SrRNA基因 序列 |
| 文章编号： | 1672-1574(2004)01-043-05 |
| 修稿时间： | 2003年4月25日 |
A Preliminary Study of Mitochondrial DNA 12S rRNA Gene Sequence of Charybdis japonica and Thalamita prymna |
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| GAO Tian-xiang,ZHANG Xiu-mei,WATANABE Seiichi,FUSEYA Reiko.A Preliminary Study of Mitochondrial DNA 12S rRNA Gene Sequence of Charybdis japonica and Thalamita prymna[J].Periodical of Ocean University of China,2004,34(1):43-47. |
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| Authors: | GAO Tian-xiang ZHANG Xiu-mei WATANABE Seiichi FUSEYA Reiko |
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| Institution: | GAO Tian-xiang1,ZHANG Xiu-mei1,WATANABE Seiichi2,FUSEYA Reiko2 |
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| Abstract: | This paper deals with the PCR amplification and sequencing of mitoch-ondrial DNA 12S rRNA gene of Charybdis japonica and Thalamita prymna. The results showed that the sequence lengths of 12S rRNA gene fragments of two species were 414bp and 415bp respectively. The A, T, G and C contents of Charybdis japonica and Thalamita prymna were 151bp (36.47%), 153bp (36.96%), 43bp (10.39%), 67bp (16.18%) and 150bp (36.14%), 155bp (37.35%), 44bp (10.60%), 66bp ( 15.90%) respectively. Between Charybdis japonica and Thalamita prymna, there were three deletions/insertions and 38bp different sites, the ratio of transition and transversion was approximately 2.17. |
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| Keywords: | Charybdis japonica Thalamita prymna 12S rRNA gene sequence |
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