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| Phylogenetic diversity of bacteria associated with the marine sponge Agelas robusta from South China Sea | |
| 作者姓名： | SUN Wei DAI Shikun WANG Guanghu XIE Lianwu JIANG Shumei LI Xiang |
| 作者单位： | [1]Key Laboratory of Marine Bio-resources Sustainable Utilization （LMB-CAS）, Guangdong Key Laboratory of Marine Materia Mediea （LMMM-GD）, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China [2]Graduate University of the Chinese Academy of Sciences, Beijing 100049, China [3]Central South University of Forestry and Technology, Changsha 410004, China |
| 基金项目： | This research was partially funded by the State Principal and Basic Research and Development Program of the Ministry of Sciences and Technology of China under contract No. 2010CB833801; the Provincial Collaborative Foundation Project of Guangdong under contract Nos 9351007002000001 and 2008A030203004. |
| 摘 要： | It is well known that marine sponges harbor large numbers of bacteria, some of which have been proved to be sponge-specific. The diversity of bacteria in marine sponges distributed along the coast of South China Sea has been previously studied but that of bacteria in sponges inhabiting the open sea has been rarely investigated. In this report, the diversity of bacteria associated with the marine sponge Agelas robusta from a remote coral reef in the South China Sea was documented employing 16S rDNA library construction, amplified ribosomal DNA restriction analysis (ARDRA) and phylogenetic analysis. A total of 32 phylotypes were finally categorized in nine phyla including Cyanobacteria, Proteobacteria, Chloroflexi, Firmicutes, Actinobacteria, Acidobacteria, Planctomycetes, Bacteroidetes and Gemmatimonadetes. The dominant phylum was Proteobacteria whereas the dominant genus was Synechococcus in Cyanobacteria. Some spongespecific bacteria were also found in the bacteria population, but the proportion (5 OTUs/32 OTUs) was much lower than other sponges. This study reveals the phylogenetic diversity of bacteria in A. robusta and confirms the presence of some sponge-specific bacteria in the South China Sea. Understanding the diversity of sponge-associated bacteria in China assists to exploit the bacteria resources for biotechnology. |
| 关 键 词： | 系统发育多样性 细菌多样性 中国沿岸 海绵 海洋 Sea 塔 系统发育分析 |
| 收稿时间： | 12 July 2006 |
| 修稿时间： | 2010/5/11 0:00:00 |
Phylogenetic diversity of bacteria associated with the marine sponge Agelas robusta from South China Sea |
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| SUN Wei,DAI Shikun,WANG Guanghu,XIE Lianwu,JIANG Shumei,LI Xiang.Phylogenetic diversity of bacteria associated with the marine sponge Agelas robusta from South China Sea[J].Acta Oceanologica Sinica,2010,29(5):65-73. | |
| Authors: | SUN Wei DAI Shikun WANG Guanghu XIE Lianwu JIANG Shumei and LI Xiang |
| Institution: | 1. Key Laboratory of Marine Bio-resources Sustainable Utilization(LMB-CAS),Guangdong Key Laboratory of Marine Materia Medica(LMMM-GD),South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301,China;Graduate University of the Chinese Academy of Sciences,Beijing 100049,China 2. Key Laboratory of Marine Bio-resources Sustainable Utilization(LMB-CAS),Guangdong Key Laboratory of Marine Materia Medica(LMMM-GD),South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301,China 3. Central South University of Forestry and Technology,Changsha 410004,China |
| Abstract: | It is well known that marine sponges harbor large numbers of bacteria, some of which have been proved to be sponge-specific. The diversity of bacteria in marine sponges distributed along the coast of South China Sea has been previously studied but that of bacteria in sponges inhabiting the open sea has been rarely investigated. In this report, the diversity of bacteria associated with the marine sponge Agelas robusta from a remote coral reef in the South China Sea was documented employing 16S rDNA library construction, amplified ribosomal DNA restriction analysis (ARDRA) and phylogenetic analysis. A total of 32 phylotypes were finally categorized in nine phyla including Cyanobacteria; Proteobacteria; Chloroflexi; Firmicutes; Actinobacteria; Acidobacteria; Planctomycetes; Bacteroidetes and Gemmatimonadetes. The dominant phylum was Proteobacteria whereas the dominant genus was Synechococcus in Cyanobacteria. Some spongespecific bacteria were also found in the bacteria population, but the proportion (5 OTUs/32 OTUs) was much lower than other sponges. This study reveals the phylogenetic diversity of bacteria in A. robusta and confirms the presence of some sponge-specific bacteria in the South China Sea. Understanding the diversity of sponge-associated bacteria in China assists to exploit the bacteria resources for biotechnology. |
| Keywords: | South China Sea Agelas robusta phylogenetic diversity sponge-specific bacteria |
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