西北地区红砂种群ISSR遗传变异的空间自相关分析

 运用空间自相关分析,对我国西北地区28个红砂种群的60个ISSR位点进行空间分布的研究,从14个ISSR引物扩增的多态位点中,选取基因频率在30％~70％之间的位点,根据等样对频率和等距离间隔的方法对红砂种群进行空间结构的分析。通过Moran’s I值显示:红砂种群的多数ISSR位点在种群间的分布缺乏一定的空间结构,为随机分布,少数ISSR位点表现出微弱的空间结构,红砂种群总体的空间结构为随机分布。红砂种群遗传变异空间模式的形成是基因流、自然选择、种群隔离以及物种扩散特性综合作用的结果。对红砂种群的空间结构分析能有助于进一步探讨红砂随机大种群的进化与生态过程,为以后的保护工作提供科学依据。

Spatial Autocorrelation Analysis of ISSR Genetic Variation of Reaumuria soongorica Population in Northwest of China

The spatial distribution of 28 Reaumuria soongorica populations in northwest of China were studied with the spatial autocorrelation analysis. The polymorphic bands with frequencies ranging from 30% to 70%, which were generated from 14 ISSR selective primers, were chosen. The approach of equal gene frequency correlograms and equal distance interval correlograms was used to analyze the spatial structure. Morans I values showed a lack of spatial structure of genetic variation existing in the population of Reaumuria soongorica. Most of the ISSR locations were randomly distributed. A few of ISSR locations had a faint spatial structure, in other words, Reaumuria soongorica populations are randomly distributed. The spatial patterns of genetic variation in Reaumuria soongorica population should be the combining result from gene flow, natural selection, population isolation and breeding mechanism. Furthermore, the further analyzing on mechanism and cause of spatial structure formation can help to discuss the evolution and ecology processes of randomly distributed Reaumuria soongorica populations, which may provide effective scientific bases for conservation work.