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| 水产养殖用芽孢杆菌制剂的检测 | |
| 引用本文： | 温崇庆,薛明,张良军,李兴,吴勇.水产养殖用芽孢杆菌制剂的检测[J].广东海洋大学学报,2011,31(1). |
| 作者姓名： | 温崇庆 薛明 张良军 李兴 吴勇 |
| 作者单位： | 1. 广东海洋大学水产学院,广东湛江,524025 2. 湛江市科壹水产养殖有限公司,广东湛江514073;湛汀粤海水产种苗有限公司,广东湛江524017 3. 湛江市科壹水产养殖有限公司,广东湛江,514073 |
| 基金项目： | 广东省海洋渔业科技推广专项(A200808A04) |
| 摘 要： | 通过平板菌落计数法检测编号为GD1、GD2、GD3、GD4、GD5、JS、JX、SD1和SD2等9种水产养殖用芽孢杆菌制剂的有效活菌数,结合形态、生理生化和16S rRNA基因序列分析对各制剂中分离的产芽孢细菌代表菌株进行鉴定。结果显示:除GD1、GD2和GD5制剂未标明芽孢数量外,其余制剂检测到的芽孢数量均低于或远低于标注数量;经鉴定,各制剂中最优势类型,GD2、GD3、GD5、SD1和SD2制剂为地衣芽孢杆菌(Bacillus licheniformis),GD4、JS和JX制剂为解淀粉芽孢杆菌(B.amyloliquefaciens),GD1制剂为枯草芽孢杆菌(B.subtilis)。除GD4、GD5和SD1制剂未标明具体菌种以及GD2的标注菌种与检测结果不一致外,其他制剂的标注菌种与被鉴定的优势类型芽孢杆菌基本一致。 |
| 关 键 词： | 芽孢杆菌 制剂 数量 鉴定 水产养殖 |
Detection of Bacillus Preparations Used for Aquaculture |
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| WEN Chong-qing,XUE Ming,ZHANG Liang-jun,LI xing,WU Yong.Detection of Bacillus Preparations Used for Aquaculture[J].Journal of Zhanjiang Ocean University,2011,31(1). | |
| Authors: | WEN Chong-qing XUE Ming ZHANG Liang-jun LI xing WU Yong |
| Institution: | WEN Chong-qing1,XUE Ming1,ZHANG Liang-jun2,3,LI xing2,WU Yong2(1.Fisheries College,Guangdong Ocean University,Zhanjiang 524025,China,2.Zhanjiang Keyi Aquaculture Co.,Ltd,Zhanjiang,514073,3.Zhanjiang Yuehai Aquatic Seed Co.Ltd,Zhangjiang,524017,China) |
| Abstract: | The numbers of viable bacteria of nine Bacillus preparations,numbered GD1,GD2,GD3,GD4,GD5,JS,JX,SD1 and SD2,used for aquaculture were determined by plate colony counts,and representative strains of spore-forming bacteria isolated from each preparation were identified based on morphological,physiological and biochemical characteristics,as well as on 16S rRNA gene sequence analysis.The results showed that the spore numbers counted in six of the nine preparations were less or much less than that of the declare... |
| Keywords: | Bacillus preparation number identification aquaculture |
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