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| 异育银鲫(Carassius auratus gibelio)源醋酸钙不动杆菌表型及分子鉴定 | |
| 引用本文： | 夏 飞,梁利国,顾 伟,谢 骏. 异育银鲫(Carassius auratus gibelio)源醋酸钙不动杆菌表型及分子鉴定[J]. 海洋与湖沼, 2013, 44(3): 801-806 |
| 作者姓名： | 夏 飞 梁利国 顾 伟 谢 骏 |
| 作者单位： | 1. 中国水产科学研究院淡水渔业研究中心农业部淡水渔业和种质资源利用重点实验室 无锡214081;南京农业大学无锡渔业学院 无锡214081 2. 中国水产科学研究院淡水渔业研究中心农业部淡水渔业和种质资源利用重点实验室 无锡214081 |
| 基金项目： | 现代农业产业技术体系建设专项, CARS-46-10 号 |
| 摘 要： | 采用常规的培养特征、理化特性及分子生物学的方法,对从濒死异育银鲫(Carassius auratus gibelio)血液中分离到的菌株SY-1进行了致病性、表型生物学、分子生物学及药敏试验的系统研究。结果表明,该菌株在盐度5—30、pH6—8,温度10—42℃的LB液体培养基中均能生长,具有脲酶。该菌株16SrRNA基因序列(Gen Bank登录存取号:JX164201,长度1435bp)与其它不动杆菌属菌的16SrRNA基因同源性相似性在99%—100%之间,构建系统发育树确定该菌株为醋酸钙不动杆菌(Acinetobacter calcoaceticus)。人工回感可导致异育银鲫死亡。药敏试验结果显示:对恩诺沙星、强力霉素、萘啶酸、复方新诺明、氧氟沙星等10种抗生素敏感;对羧苄青霉素、诺氟沙星、大观霉素、卡那霉素、四环素等14种抗生素中度敏感;对氨苄西林、苯唑西林、林可霉素、万古霉素、氟苯尼考等15种抗生素耐药。 |
| 关 键 词： | 异育银鲫(Carassius auratus gibelio) 醋酸钙不动杆菌(Acinetobacter calcoaceticus) 致病性 药敏试验 |
| 收稿时间： | 2012-08-23 |
| 修稿时间： | 2012-10-21 |
PHENOTYPES AND MOLECULAR IDENTIFICATION OF PATHOGENIC ACINETOBACTER CALCOACETICUS FROM CARASSIUS AURATUS GIBELIO |
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| XIA Fei,LIANG Li-Guo,GU Wei and XIE Jun. PHENOTYPES AND MOLECULAR IDENTIFICATION OF PATHOGENIC ACINETOBACTER CALCOACETICUS FROM CARASSIUS AURATUS GIBELIO[J]. Oceanologia Et Limnologia Sinica, 2013, 44(3): 801-806 | |
| Authors: | XIA Fei LIANG Li-Guo GU Wei XIE Jun |
| Affiliation: | Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences; Wuxi Fisheries College, Nanjing Agricultural University;Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences;Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences; Wuxi Fisheries College, Nanjing Agricultural University;Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences |
| Abstract: | |
| Keywords: | Carassius auratus gibelio Acinetobacter calcoaceticus pathogenicity drug sensitivity test |
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