| Characterization of marine microplankton communities of Qingdao coastal areas using randomly amplified polymorphic DNA（RAPD） |
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| 作者单位： | GAO Shan,HU Xiaozhong,CHEN Zigui,XU Henglong,YI Zhenzhen(Laboratory of Protozoology, Key Laboratory of Marieulture, Ocean University of China, Qingdao 266003, China);LIN Xiaofeng,LIJiqiu(Laboratory of Protozoology, College of Life Science, South China Normal University, Guangzhou 510631, China) |
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| 基金项目： | 国家自然科学基金，Program for New Century Excellent Talents in Universities of China |
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| 摘 要： |
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| 关 键 词： | 随机扩增多态性DNA 青岛沿海地区 RAPD 社区 海洋 表征 时间尺度 遗传多样性 |
Characterization of marine microplankton communities of Qingdao coastal areas using randomly amplified polymorphic DNA(RAPD) |
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| GAO Shan,HU Xiaozhong,CHEN Zigui,XU Henglong,YI Zhenzhen,LIN Xiaofeng,LIJiqiu. Characterization of marine microplankton communities of Qingdao coastal areas using randomly amplified polymorphic DNA(RAPD)[J]. Acta Oceanologica Sinica, 2009, 28(1) |
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| Authors: | GAO Shan HU Xiaozhong CHEN Zigui XU Henglong YI Zhenzhen LIN Xiaofeng LIJiqiu |
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| Affiliation: | 1. Laboratory of Protozoology, Key Laboratory of Marieulture, Ocean University of China, Qingdao 266003, China
2. Laboratory of Protozoology, College of Life Science, South China Normal University, Guangzhou 510631, China |
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| Abstract: | Microplankton communities of three coastal sites of Qingdao, Shandong Province, China were investigated using RAPD (random amplified polymorphic DNA) molecular markers and morphological observations. Eight RAPD-primers were selected to amplify the DNA polymorphy. The genetic distances inferred from the pairwise similari-ties were calculated for the phylogenetic tree construction. Meantime, the traditional microscopic determination, a way of visualizing the species composition, was performed to detect the major taxa of microplanktons from all samples. Results showed that: (1) the band sharing index values were in the range of 0. 504 2-0. 763 2 among samples from the same sampling site at different time scales, while 0.406 5-0.685 7 among the samples from different stations at the same time scales, indicating that spatial variations of microplankton communities were more pronounced than temporal ones; (2) samples from the same station basically clustered together, cor-responding to the geographic distribution of the sampling sites; (3) diversity derived from genetic and morpho-logical data did not correspond with each other well. |
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| Keywords: | marine plankton RAPD community diversity phylogeny |
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