| Study on extraction of agaropectin from Gelidium amansii and its anticoagulant activity |
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| 作者姓名： | 綦慧敏 李大新 张晶晶 刘力 张全斌 |
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| 作者单位： | Institute of Oceanology Chinese Academy of Sciences，Shandong Provincial Pharmaceutical Co.，Institute of Oceanology，Chinese Academy of Sciences，Institute of Oceanology，Chinese Academy of Sciences，Institute of Oceanology，Chinese Academy of Sciences，Qingdao 266071，China Weifang Medical University，Weifang 261042，China，Jinan 250014，China，Qingdao 266071，China，Qingdao 266071，China，Qingdao 266071，China |
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| 基金项目： | the Innovative Key Project of Chinese Academy of Sciences，山东省科技厅资助项目 |
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| 摘 要： | Gelidium amansii agar was fractionated on DEAE-cellulose and four fractions were obtained sequentially. The yields of 1.0 mol/L NaCl fraction and 2.5 mol/L NaCl fraction were 2.80% and 2.03%. They are highly sulfated agar, and named as agaropectin with sulfate content being 22.8% and 32.5%, respectively. The anticoagulant experiment results show that agaropectin could effectively prolong the coagulation time in a dose-dependent manner in vitro. Agaropection could be absorbed and effectively prolong the plasma coagulation time in vivo. After intragastric administration at the doses of 100, 200, and 400 mg/kg-d in rats for 15 days, TT （thrombin time）, CT （coagulation time）, PT （prothrombin time）, and APTT （activated partial thromboplastin time） could be effectively prolonged and the plasma Fib level could be significantly lowered.
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| 关 键 词： | 琼脂胶 琼脂 抗凝作用 海洋生物 |
| 收稿时间： | 14 May 2009 |
Study on extraction of agaropectin from <Emphasis Type="Italic">Gelidium amansii</Emphasis> and its anticoagulant activity |
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| Huimin Qi,Daxin Li,Jingjing Zhang,Li Liu,Quanbin Zhang.Study on extraction of agaropectin from Gelidium amansii and its anticoagulant activity[J].Chinese Journal of Oceanology and Limnology,2008,26(2):186-189. |
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| Authors: | Huimin Qi Daxin Li Jingjing Zhang Li Liu Quanbin Zhang |
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| Institution: | [1]Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China [2]Weifang Medical University, Weifang 261042, China [3]Shandong Provincial Pharmaceutical Co., Jinan 250014, China |
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| Abstract: | Gelidium amansii agar was fractionated on DEAE-cellulose and four fractions were obtained sequentially.The yields of 1.0mol/L NaCl fraction and 2.5mol/L NaCl fraction were 2.80% and 2.03%.They are highly sulfated agar,and named as agaropectin with sulfate content being 22.8% and 32.5%,respectively.The anticoagulant experiment results show that agaropectin could effectively prolong the coagulation time in a dose-dependent manner in vitro.Agaropection could be absorbed and effectively prolong the plasma coagulation time in vivo.After intragastric administration at the doses of 100,200,and 400mg/kg·d in rats for 15 days,TT (thrombin time),CT (coagulation time),PT (prothrombin time),and APTT (activated partial thromboplastin time) could be effectively prolonged and the plasma Fib level could be significantly lowered. |
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| Keywords: | agar Gelidium amansii agaropectin anticoagulation |
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