| 海胆相关菌株抗菌活性筛选及其生物学特性 |
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| 引用本文： | 黄 苛,刘祝祥,陈奇辉,贺建武,粟 银,张 丽,陈义光.海胆相关菌株抗菌活性筛选及其生物学特性[J].海洋科学,2011,35(8):25-31. |
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| 作者姓名： | 黄 苛 刘祝祥 陈奇辉 贺建武 粟 银 张 丽 陈义光 |
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| 作者单位： | 吉首大学植物资源保护与利用湖南省高校重点实验室, 生物资源与环境科学学院;吉首大学植物资源保护与利用湖南省高校重点实验室, 生物资源与环境科学学院;吉首大学植物资源保护与利用湖南省高校重点实验室, 生物资源与环境科学学院;吉首大学植物资源保护与利用湖南省高校重点实验室, 生物资源与环境科学学院;吉首大学植物资源保护与利用湖南省高校重点实验室, 生物资源与环境科学学院;吉首大学植物资源保护与利用湖南省高校重点实验室, 生物资源与环境科学学院;吉首大学植物资源保护与利用湖南省高校重点实验室, 生物资源与环境科学学院 |
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| 基金项目： | 国家自然科学基金项目(30970007) |
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| 摘 要： | 以8个菌株（3株革兰氏阳性菌、3株革兰氏阴性菌和2株真菌）作为指示菌，采用管碟法对分离自南海硇洲岛马粪海hP-（Hemicentrotuspulcherrimus）中的106株细菌（含放线菌）进行抗菌活性筛选，并对其中抗菌活性较强的菌株进行了基于16SrRNA基因序列的系统发育分析和生物学特性研究。结果显示．具有抗菌活...
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| 关 键 词： | 海胆相关细菌 抗菌活性 生物学特性 系统发育 |
| 收稿时间： | 2010/10/12 0:00:00 |
| 修稿时间： | 2011/1/23 0:00:00 |
Screening and biological characteristics of antibiotic- producing bacteria from sea urchins |
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| HUANG Ke,LIU Zhu-xiang,CHEN Qi-hui,HE Jian-wu,SU Yin,ZHANG Li,CHEN Yi-guang.Screening and biological characteristics of antibiotic- producing bacteria from sea urchins[J].Marine Sciences,2011,35(8):25-31. |
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| Authors: | HUANG Ke LIU Zhu-xiang CHEN Qi-hui HE Jian-wu SU Yin ZHANG Li CHEN Yi-guang |
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| Institution: | HUANG Ke,LIU Zhu-xiang,CHEN Qi-hui,HE Jian-wu,SU Yin,ZHANG Li,CHEN Yi-guang(Key Laboratory of Plant Resources Conservation and Utilization of Hunan Province,College of Biology and En-vironment Science,Jishou University,Jishou 416000,China) |
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| Abstract: | The antimicrobial activity of 106 marine bacteria, isolated from sea urchins (Hemicentrotus pulcherrimus) collected from the coastal area of the Naozhou island in the South China Sea, were screened using the cylinder-plate method on 8 indicator microorganisms, i.e. three Gram-positive bacteria (Bacillus subtilis, Sarcina lutea, Staphylococcus aureus), three Gram-negative bacteria (Aerobacter aerogenes, Escherichia coli, Proteus vulgaris), and two fungi (Asperillus niger, Candida albican). Out of 106 test strains, 59 were positive in antimicrobial activity (55.7%), in which 10 strains exhibited strong antimicrobial activity. These 10 strains were submitted for 16S rRNA gene amplification and phylogenetic analysis after morphological, biochemical, and physiological characterization. It was found that the 10 strains were within the phyla Firmicutes and Proteobacteria, belong to the genera Halobacillus (JSM 076029, JSM 076017, JSM 076062), Bacillus (JSM 076053, JSM 076009), Pontibacillus (JSM 076056), Planococcus (JSM 076050), Jeotgalibacillus (JSM 076100), Salinicoccus (JSM 076102), and Serratia (JSM 076077). |
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| Keywords: | Associated bacteria with sea urchin antimicrobial activity biological characteristics phylogenetic analysis |
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