| Fatty Acid Composition Analyses of the DCMU Resistant Mutants of Nannochloropsis oculata (Eustigmatophyceae) |
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| 作者姓名： | ZHANG Jimin LIU Shuang SUN Xue YANG Guanpin ZHANG Xuecheng * GAO Zhenhui North China Sea Monitoring Center SOA Fushun Road Qingdao P..R.China College of Marine Life Sciences Ocean University of China Qingdao P..R.China |
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| 作者单位： | ZHANG Jimin 1)，LIU Shuang 2)，SUN Xue 2)，YANG Guanpin 2)，ZHANG Xuecheng 2)，*，GAO Zhenhui 1) 1) North China Sea Monitoring Center，SOA，22 Fushun Road，Qingdao 266033，P..R.China2) College of Marine Life Sciences，Ocean University of China，Qingdao 266003，P..R.China |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 1 Introduction Nannochloropsissp .areoneofpotentialsourcesofpolyunsaturatedfattyacids ,especiallytheeicosapen taentaenoicacid (EPA ,C2 0∶5 ) ,whichisimportantforbothanimalsandhumans . Thefattyacidcompositionofthisalgaisaffectedbyvariousenvironmentalfactors ,suchaslightintensity(Sukeniketal.,1989;Sukeniketal.,1990 ) ,imply ingthatafactorinfluencingthephotosyntheticprocessmayaffectcellularfattyacidsynthesisandtheirmetabolism .AselectionprogramofincreasingcellularEPAcontenthasbeencarriedo…
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Fatty Acid Composition Analyses of the DCMU Resistant Mutants of Nannochloropsis oculata (Eustigmatophyceae) |
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| ZHANG Jimin ,LIU Shuang ,SUN Xue ,YANG Guanpin ,ZHANG Xuecheng ,*,GAO Zhenhui North China Sea Monitoring Center,SOA, Fushun Road,Qingdao ,P..R.China College of Marine Life Sciences,Ocean University of China,Qingdao ,P..R.China.Fatty Acid Composition Analyses of the DCMU Resistant Mutants of Nannochloropsis oculata (Eustigmatophyceae)[J].Journal of Ocean University of China,2003,2(1). |
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| Authors: | Zhang Jimin LIU Shuang SUN Xue Yang Guanpin ZHANG Xuecheng GAO Zhenhui |
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| Abstract: | Ultraviolet mutagenesis was applied to Nannochloropsis oculata and three mutants resistant to 3-(3, 4-dichlorophenyl)-1, 1-dimethylurea (DCMU) were isolated. The cellular chlorophyll a and total lipid content of the wild are higher in the medium supplemented with DCMU than in the control without DCMU. Without DCMU, the growth rates and chlorophyll a contents of the mutants are similar to those of the wild. Significant changes of fatty acid content and composition have occurred in DCMU-resistant mutants growing in the medium supplemented with DCMU. The total lipid, palmitic acid (16:0), palmitoleic acid (16:1ω9) and oleic (18:1ω9) contents decrease significantly, while the vaccenic acid (18:1ω11) increases significantly and the EPA content of dried powder increases slightly in the mutants. The study may provide a basis to improve EPA content in Nannochloropsis oculata in the future. |
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| Keywords: | Nannochloropsis oculata DCMU mutation fatty acid |
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