基于MALDI-TOF MS快速鉴定弧菌的数据库的扩展和应用

近年来由海洋细菌导致的感染越来越常见，弧菌是海洋细菌中最常见的细菌之一。目前，对弧菌缺乏快速有效的鉴定。基质辅助激光解吸电离飞行时间质谱仪（Matrix-assisted laser desorption ionization–time of flight mass spectrometry，MALDI-TOF MS）已成为快速鉴别细菌强有力的工具。然而，它的准确性取决于数据库中的菌株数量。本研究中，我们利用MALDI-TOF MS对弧菌数据库进行了扩展和应用。我们将74株弧菌纳入了新的数据库，构建了基于RpoB序列的进化树（邻接法和最大似然比法），并针对扩展后数据库和扩展前数据库进行了聚类分析。我们利用30株弧菌，对扩展后数据库进行了评价，并进行了主成分分析。本研究中，我们创建了一个新的数据库，促进了弧菌的快速准确鉴定。MSP(main spectrum profile)和PCA（Principal Component Analysis）分析为追踪弧菌感染的来源和发生率提供了理论支持。此外，特征峰和差异峰的发现对弧菌的鉴定也有帮助。

Extension and application of a database for the rapid identification of Vibrio using MALDI-TOF MS

Rapid and accurate identification of Vibrio species has been problematic because phenotypic characteristics are variable within species and biochemical identification requires two or more days to complete. Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) has become a powerful tool for rapidly distinguishing between related bacterial species. However, its accuracy depends on the number of strains in a database. In the current study, we extend and apply the Vibrio database based on MALDI-TOF MS. A total of 74 strains of Vibrio representing 28 species were identified and included in new database. A phylogenetic tree based on rpoB sequence and dendrograms were constructed. We analyzed 30 clinical Vibrio of three species to evaluate the new database and carried out PCA dendrogram analyses for differences of strains. We created a new database that offered fast and accurate Vibrio identification. MSP and PCA dendrogram analyses provided technical support to track sources and incidences of Vibrio infection. In addition, the discovery of characteristic and differential peaks is useful for the future identification of Vibrio. This represents a powerful tool for the rapid and accurate classification and identification of Vibrio and closely related species.